Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

SARS-CoV-2 in Wastewater: Occurrence, Detection and Implications

Version 1 : Received: 1 April 2022 / Approved: 4 April 2022 / Online: 4 April 2022 (11:04:30 CEST)

How to cite: Billa, A.; Balda, S.; Sharma, A.; Dhingra, R.; Sah, S.N.; Capalash, N.; Sharma, P. SARS-CoV-2 in Wastewater: Occurrence, Detection and Implications. Preprints 2022, 2022040015. https://doi.org/10.20944/preprints202204.0015.v1 Billa, A.; Balda, S.; Sharma, A.; Dhingra, R.; Sah, S.N.; Capalash, N.; Sharma, P. SARS-CoV-2 in Wastewater: Occurrence, Detection and Implications. Preprints 2022, 2022040015. https://doi.org/10.20944/preprints202204.0015.v1

Abstract

Coronavirus Disease-19 (COVID-19) is presently wreaking havoc on public health and socio-economic development. Besides the upper and lower respiratory tract involvement, gastrointestinal symptoms are also reported in COVID-19 patients through gut-lung axis. Finding its way through the feces of infected individuals and other sources, the genetic material of SARS-CoV-2 (ssRNA) is reported widely in wastewater and is being used as a fingerprint for its detection. With millions of cases arriving every day, there is a need to level up the testing speed efficiency. Due to the restricted sampling potential of testing laboratories, clinical testing is unable to track all the symptomatic and asymptomatic cases. Wastewater-based epidemiology (WBE) bestows an auxiliary monitoring tool that will contribute in community level screening. Sample collection, concentration, RNA extraction, quantification and data analysis are the main steps involved in implementation of WBE that can be relied upon as an alarm call for an upcoming wave, emergence of a new variant or any future pandemic. WBE can be a cheaper and more practical alternative to high end and sophisticated clinical testing for community transmission detection. Worldwide, there are more than 300 reports entailing the occurrence of SARS-CoV-2 in wastewater exhibiting unique temporal trends with five of them in India. This review aims to address the present knowledge on surveillance of SARS-CoV-2 in wastewater and its implications.

Keywords

Wastewater; Surveillance; SARS-CoV-2; Wastewater based epidemiology; COVID-19; Detection; Sewage

Subject

Biology and Life Sciences, Immunology and Microbiology

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