Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites

Version 1 : Received: 29 March 2022 / Approved: 1 April 2022 / Online: 1 April 2022 (10:21:11 CEST)

How to cite: Clavo, R.F.; Pereira, A.K.; Quiñones, N.R.; Costa, J.H.; Fill, T.P.; Garboggini, F.F. Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites. Preprints 2022, 2022040005 (doi: 10.20944/preprints202204.0005.v1). Clavo, R.F.; Pereira, A.K.; Quiñones, N.R.; Costa, J.H.; Fill, T.P.; Garboggini, F.F. Metabolomic Comparison Using Streptomyces spp as a Factory of Secondary Metabolites. Preprints 2022, 2022040005 (doi: 10.20944/preprints202204.0005.v1).

Abstract

Understanding extremophiles and their usefulness in biotechnology involves studying their habitat, physiology and biochemical adaptations , as well as their ability to produce biocatalysts, in environments that are still poorly explored. In northwestern Peru, which saline lagoons of marine origin Pacific Ocean, the other site from the coast of Brazil of the Atlantic Ocean. Both environments are considered extreme. The objective of the present work was to compare two different strains isolated from these extreme environments at the metabolic level using molecular network methodology through the Global Natural Products Molecular Social Network (GNPS). In our study, the MS/MS spectra from the network were compared with GNPS spectral libraries, where the metabolites were annotated. Differences were observed in the molecular network presented in the two strains of Streptomyces spp. coming from these two different environments. Within the annotated compounds from marine bacteria, the metabolites characterized for Streptomyces sp. B-81 from Peruvian marshes were lobophorins A (1) and H (2), as well as divergolides A (3), B (4) and C (5). Streptomyces sp. 796.1 produced different compounds, such as glucopiericidin A (6) and dehydro-piericidin A1a (7). The search for new metabolites in underexplored environments may therefore reveal new metabolites with potential application in different areas of biotechnology.

Keywords

genome mining; marine environments; molecular networking; bacterial extremophiles; secondary metabolites

Subject

MEDICINE & PHARMACOLOGY, Other

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