Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds

Version 1 : Received: 27 February 2022 / Approved: 3 March 2022 / Online: 3 March 2022 (10:31:32 CET)

How to cite: Seidel, C.; Birkemeyer, C.; Baran-Schmidt, R.; Meixensberger, J.; Oppermann, H.; Gaunitz, F. Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds. Preprints 2022, 2022030060 (doi: 10.20944/preprints202203.0060.v1). Seidel, C.; Birkemeyer, C.; Baran-Schmidt, R.; Meixensberger, J.; Oppermann, H.; Gaunitz, F. Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds. Preprints 2022, 2022030060 (doi: 10.20944/preprints202203.0060.v1).

Abstract

The naturally occurring dipeptide carnosine (β alanyl L histidine) specifically attenuates tumor growth. Here, we asked whether other small imidazole containing compounds also affect viability of tumor cells without affecting non-malignant cells, and whether formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L alanyl L histidine (LA-LH), ß alanyl L alanine, L histidine, histamine, imidazole, β alanine and L alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L histidine and formation of histamine was investigated by High Performance Liquid Chromatography coupled Mass Spectrometry. Whereas carnosine and LA LH inhibited tumor cell growth with minor effects on fibroblasts, L-histidine, histamine and imidazole affected viability in both cell types. Compounds without imidazole moiety did not diminish viability. In the presence of LA LH but not in the presence of carnosine a significant rise of intracellular amounts of histidine was detected in all cells. Formation of histamine was not detectable in the presence of carnosine, LA LH or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA LH. Despite histamine had a strong effect on cell viability, formation of histamine is not responsible for the effects on cell viability of carnosine, LA LH and histidine.

Keywords

carnosine; glioblastoma; fibroblasts; imidazole-containing compounds; cell viability; high per-formance liquid chromatography coupled mass spectrometry

Subject

MEDICINE & PHARMACOLOGY, Oncology & Oncogenics

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.