Version 1
: Received: 27 February 2022 / Approved: 3 March 2022 / Online: 3 March 2022 (10:31:32 CET)
How to cite:
Seidel, C.; Birkemeyer, C.; Baran-Schmidt, R.; Meixensberger, J.; Oppermann, H.; Gaunitz, F. Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds. Preprints2022, 2022030060. https://doi.org/10.20944/preprints202203.0060.v1.
Seidel, C.; Birkemeyer, C.; Baran-Schmidt, R.; Meixensberger, J.; Oppermann, H.; Gaunitz, F. Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds. Preprints 2022, 2022030060. https://doi.org/10.20944/preprints202203.0060.v1.
Cite as:
Seidel, C.; Birkemeyer, C.; Baran-Schmidt, R.; Meixensberger, J.; Oppermann, H.; Gaunitz, F. Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds. Preprints2022, 2022030060. https://doi.org/10.20944/preprints202203.0060.v1.
Seidel, C.; Birkemeyer, C.; Baran-Schmidt, R.; Meixensberger, J.; Oppermann, H.; Gaunitz, F. Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazol-Containing Compounds. Preprints 2022, 2022030060. https://doi.org/10.20944/preprints202203.0060.v1.
Abstract
The naturally occurring dipeptide carnosine (β alanyl L histidine) specifically attenuates tumor growth. Here, we asked whether other small imidazole containing compounds also affect viability of tumor cells without affecting non-malignant cells, and whether formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L alanyl L histidine (LA-LH), ß alanyl L alanine, L histidine, histamine, imidazole, β alanine and L alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L histidine and formation of histamine was investigated by High Performance Liquid Chromatography coupled Mass Spectrometry. Whereas carnosine and LA LH inhibited tumor cell growth with minor effects on fibroblasts, L-histidine, histamine and imidazole affected viability in both cell types. Compounds without imidazole moiety did not diminish viability. In the presence of LA LH but not in the presence of carnosine a significant rise of intracellular amounts of histidine was detected in all cells. Formation of histamine was not detectable in the presence of carnosine, LA LH or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA LH. Despite histamine had a strong effect on cell viability, formation of histamine is not responsible for the effects on cell viability of carnosine, LA LH and histidine.
Keywords
carnosine; glioblastoma; fibroblasts; imidazole-containing compounds; cell viability; high per-formance liquid chromatography coupled mass spectrometry
Subject
MEDICINE & PHARMACOLOGY, Oncology & Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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