Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Version 1 : Received: 26 February 2022 / Approved: 1 March 2022 / Online: 1 March 2022 (11:13:07 CET)

A peer-reviewed article of this Preprint also exists.

Bogusiewicz, J.; Kupcewicz, B.; Goryńska, P.Z.; Jaroch, K.; Goryński, K.; Birski, M.; Furtak, J.; Paczkowski, D.; Harat, M.; Bojko, B. Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes. Int. J. Mol. Sci. 2022, 23, 3518. Bogusiewicz, J.; Kupcewicz, B.; Goryńska, P.Z.; Jaroch, K.; Goryński, K.; Birski, M.; Furtak, J.; Paczkowski, D.; Harat, M.; Bojko, B. Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes. Int. J. Mol. Sci. 2022, 23, 3518.

Journal reference: Int. J. Mol. Sci. 2022, 23, 3518
DOI: 10.3390/ijms23073518

Abstract

The development of a fast and accurate intraoperative method that enables the differentiation and stratification of cancerous lesions is still a challenging problem in laboratory medicine. Therefore, it is important to find and optimize a simple and effective analytical method that enables the selection of distinctive metabolites. This study aims to assess the usefulness of solid-phase microextraction (SPME) probes as a sampling method for the lipidomic analysis of brain tumors. To this end, SPME was applied to sample brain tumors immediately after excision, followed by lipidomic analysis via liquid chromatography-high resolution mass spectrometry (LC-HRMS). The results showed that long fibers were a good option for extracting analytes from an entire lesion to obtain an average lipidomic profile. Moreover, significant differences between tumors of different histological origin were observed. In-depth investigation of the glioma samples revealed that malignancy grade and isocitrate dehydrogenase (IDH) mutation status impact the lipidomic composition of the tumor, whereas 1p/19q co-deletion did not appear to alter the lipid profile. This first on-site lipidomic analysis of intact tumors proved that chemical biopsy with SPME is a promising tool for the simple and fast extraction of lipid markers in neurooncology.

Keywords

brain tumor; glioma; SPME; heterogeneity; lipids; chemical biopsy

Subject

MEDICINE & PHARMACOLOGY, Oncology & Oncogenics

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