Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Joanna Bogusiewicz
ORCID logo ,
Bogumiła Kupcewicz
ORCID logo ,
Paulina Zofia Goryńska
,
Karol Jaroch
ORCID logo ,
Krzysztof Goryński
,
Marcin Birski
ORCID logo ,
Jacek Furtak
,
Dariusz Paczkowski
,
Marek Harat
,
Barbara Bojko
* ORCID logo
Version 1 : Received: 26 February 2022 / Approved: 1 March 2022 / Online: 1 March 2022 (11:13:07 CET)

A peer-reviewed article of this Preprint also exists.

Bogusiewicz, J.; Kupcewicz, B.; Goryńska, P.Z.; Jaroch, K.; Goryński, K.; Birski, M.; Furtak, J.; Paczkowski, D.; Harat, M.; Bojko, B. Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes. Int. J. Mol. Sci. 2022, 23, 3518. Bogusiewicz, J.; Kupcewicz, B.; Goryńska, P.Z.; Jaroch, K.; Goryński, K.; Birski, M.; Furtak, J.; Paczkowski, D.; Harat, M.; Bojko, B. Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes. Int. J. Mol. Sci. 2022, 23, 3518.

Abstract

The development of a fast and accurate intraoperative method that enables the differentiation and stratification of cancerous lesions is still a challenging problem in laboratory medicine. Therefore, it is important to find and optimize a simple and effective analytical method that enables the selection of distinctive metabolites. This study aims to assess the usefulness of solid-phase microextraction (SPME) probes as a sampling method for the lipidomic analysis of brain tumors. To this end, SPME was applied to sample brain tumors immediately after excision, followed by lipidomic analysis via liquid chromatography-high resolution mass spectrometry (LC-HRMS). The results showed that long fibers were a good option for extracting analytes from an entire lesion to obtain an average lipidomic profile. Moreover, significant differences between tumors of different histological origin were observed. In-depth investigation of the glioma samples revealed that malignancy grade and isocitrate dehydrogenase (IDH) mutation status impact the lipidomic composition of the tumor, whereas 1p/19q co-deletion did not appear to alter the lipid profile. This first on-site lipidomic analysis of intact tumors proved that chemical biopsy with SPME is a promising tool for the simple and fast extraction of lipid markers in neurooncology.

Keywords

brain tumor; glioma; SPME; heterogeneity; lipids; chemical biopsy

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.