Preprint Technical Note Version 1 Preserved in Portico This version is not peer-reviewed

Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi

Version 1 : Received: 24 February 2022 / Approved: 25 February 2022 / Online: 25 February 2022 (15:26:33 CET)

How to cite: Soto-Àngel, J.J.; Nordmann, E.; Sturm, D.; Sachkova, M.; Pang, K.; Burkhardt, P. Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi. Preprints 2022, 2022020341 (doi: 10.20944/preprints202202.0341.v1). Soto-Àngel, J.J.; Nordmann, E.; Sturm, D.; Sachkova, M.; Pang, K.; Burkhardt, P. Stable Laboratory Culture System for the Ctenophore Mnemiopsis leidyi. Preprints 2022, 2022020341 (doi: 10.20944/preprints202202.0341.v1).

Abstract

Ctenophores are marine organisms attracting significant attention from evolutionary, molecular biology and ecological research. Here we describe an easy and affordable set-up to maintain a stable culture of the ctenophore Mnemiopsis leidyi. The challenging delicacy of the lobate ctenophores can be met by monitoring the water quality, providing the right nutrition, and adapting the handling and tank set-up to their fragile gelatinous body plan. Following this protocol allows stable laboratory lines, a continuous supply of embryos for molecular biological studies, and independence from population responses to environmental fluctuations.

Keywords

Ctenophora; breeding; comb jelly; cultivation; husbandry; protocol; sea walnut

Subject

BIOLOGY, Animal Sciences & Zoology

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