Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Characterization of Bifidobacterium asteroides Isolates

Version 1 : Received: 8 February 2022 / Approved: 11 February 2022 / Online: 11 February 2022 (21:17:45 CET)

A peer-reviewed article of this Preprint also exists.

Pino, A.; Benkaddour, B.; Inturri, R.; Amico, P.; Vaccaro, S.C.; Russo, N.; Vaccalluzzo, A.; Agolino, G.; Caggia, C.; Miloud, H.; Randazzo, C.L. Characterization of Bifidobacterium asteroides Isolates. Microorganisms 2022, 10, 655. Pino, A.; Benkaddour, B.; Inturri, R.; Amico, P.; Vaccaro, S.C.; Russo, N.; Vaccalluzzo, A.; Agolino, G.; Caggia, C.; Miloud, H.; Randazzo, C.L. Characterization of Bifidobacterium asteroides Isolates. Microorganisms 2022, 10, 655.

Journal reference: Microorganisms 2022, 10, 655
DOI: 10.3390/microorganisms10030655

Abstract

Bifidobacteria have long been recognized as bacteria with probiotic and therapeutic features. The aim of this work is to characterize the Bifidobacterium asteroides BA15 and BA17 strains, isolated from honeybee gut. An in-depth assessment was carried out on safety properties (antibiotic resistance profiling, β-haemolytic, DNAse and gelatinase activities and virulence factor presence) and other properties (antimicrobial activity, auto-aggregation, co-aggregation and hydrophobicity). Based on phenotypic and genotypic characterization, both strains satisfied all the safety requirements. More specifically, genome analysis showed the absence of genes encoding for glycopeptide (vanA, vanB, vanC-1, vanC-2, vanD, vanE, vanG), resistance to tetracycline (tet-M, tet-L and tetO), and virulence genes (asa1, gelE, cylA, esp, hyl).

Keywords

microbiological characterization; safety; VanZ; isolation; vancomycin resistant gene; genome; bee; honey

Subject

LIFE SCIENCES, Microbiology

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