Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Differential Acellular Reactive Oxygen Species (ROS) Generation by E-cigarettes Containing Tobacco-Free Nicotine (TFN) and Tobacco-Derived Nicotine

Version 1 : Received: 7 February 2022 / Approved: 9 February 2022 / Online: 9 February 2022 (10:37:28 CET)

A peer-reviewed article of this Preprint also exists.

Yogeswaran, S.; Rahman, I. Differences in Acellular Reactive Oxygen Species (ROS) Generation by E-Cigarettes Containing Synthetic Nicotine and Tobacco-Derived Nicotine. Toxics 2022, 10, 134. Yogeswaran, S.; Rahman, I. Differences in Acellular Reactive Oxygen Species (ROS) Generation by E-Cigarettes Containing Synthetic Nicotine and Tobacco-Derived Nicotine. Toxics 2022, 10, 134.

Journal reference: Toxics 2022, 10, 134
DOI: 10.3390/toxics10030134

Abstract

Tobacco Free Nicotine (TFN) Electronic Nicotine Delivery Systems (ENDS) are yet to be classified as tobacco products; consequently, there is ambiguity over whether Food and Drug Administration (FDA) regulatory authority can be extended to include TFN e-cigarettes. In recent years, a more significant number of e-cigarette companies have been manufacturing TFN-containing e-cigarettes and e-liquids to circumvent FDA regulations. While studies have shown that aerosols generated from tobacco-derived nicotine-containing e-cigarettes contain significant Reactive Oxygen Species (ROS) levels, no comparison studies have been conducted using TFN e-cigarettes. This study uses a single puff aerosol generator to aerosolize TFN and tobacco-derived nicotine-containing vape-products and subsequently involves semi-quantifying ROS generated by these vape products in H2O2 equivalents. We found that the differences between ROS levels generated from TFN and tobacco-derived nicotine-containing vape products vary by flavor. TFN tobacco flavors and fruit flavors are more toxic in terms of ROS generation than menthol/ice and drink/beverage flavored products using TFN. The type of salt nicotine used in vape products, TFN or tobacco-derived nicotine, impacts the level of ROS generated from flavored vape products. Thus, a better understanding of the type of salt nicotine used in e-cigarettes (tobacco-derived or synthetic), has modulating effect on lung oxidative stress.

Keywords

Tobacco Free Nicotine (TFN); Synthetic Nicotine; Tobacco-derived Nicotine; vape-bar; Electronic Nicotine Delivery Systems; Reactive Oxygen Species (ROS)

Subject

MEDICINE & PHARMACOLOGY, Pharmacology & Toxicology

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