Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Growth, Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. And Cyanothece sp. As Affected by White and Colored Artificial Light in Batch Cultures

Version 1 : Received: 27 January 2022 / Approved: 28 January 2022 / Online: 28 January 2022 (12:21:12 CET)

How to cite: Hotos, G.N.; Antoniadis, T.I. Growth, Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. And Cyanothece sp. As Affected by White and Colored Artificial Light in Batch Cultures. Preprints 2022, 2022010437 (doi: 10.20944/preprints202201.0437.v1). Hotos, G.N.; Antoniadis, T.I. Growth, Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium sp. And Cyanothece sp. As Affected by White and Colored Artificial Light in Batch Cultures. Preprints 2022, 2022010437 (doi: 10.20944/preprints202201.0437.v1).

Abstract

Cyanobacteria are extensively studied and cultured because they can produce many value-added substances among which are pigments, mainly the phycobiliproteins phycocyanin (PC), phycoerythrin (PE), allophycocyanin (APC) and chlorophyll-a and carotenoids as well. As numerous cyanobacterial species await optimization for maximizing pigment production, we examined here two local marine species, Phormidium sp. and Cyanothece sp. batch cultured under 18-19.5 oC, at 40 ppt salinity with Walne’s nutrient medium, using white LED light of low (2000 lux) and high (8000 lux) intensity and additionally blue, green and red LED light. Significant differences were found among the intensities and colors of light used. Maximum growth was induced by high white light in both species (2.15 g dw/L in Phormidium and 1.47 g/L in Cyanothece). Next to them was green light (1.25 g/L) in Cyanothece and low white and green (1.26 – 1.33 g/L) in Phormidium. Green light maximized phycocyanin content in Phormidium (0.45 mg/mL), while phycoerythrin was maximized (0.17 mg/mL) by blue light and allophycocyanin by all colors (~0.80 mg/mL). All colors maximized phycocyanin in Cyanothece (~0.32 mg/mL) while phycoerythrin and allophycocyanin were maximized under green light (~0.138 and 0.38 mg/mL respectively). In Phormidium maximization of chlorophyll-a (9.3 μg/mL) was induced by green light while total carotenoids and b-carotene (3.05 and 0.89 μg/mL respectively) by high white light. In Cyanothece both white light intensities along with green light maximized chlorophyll-a content (~9 μg/mL) while high white light and green maximized total carotenoids (2.6-3.0 μg/mL).

Keywords

cyanobacteria; Phormidium; Cyanothece; culture growth; light; chlorophyll; carotenoids; phycocyanin; phycoerythrin; allophycocyanin; phycobiliproteins

Subject

BIOLOGY, Plant Sciences

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