Version 1
: Received: 25 January 2022 / Approved: 26 January 2022 / Online: 26 January 2022 (18:46:41 CET)
How to cite:
Xu, D.; Li, Z.; Guo, M.; Jiang, B.; Zhang, E.; Zheng, X. Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract. Preprints2022, 2022010405. https://doi.org/10.20944/preprints202201.0405.v1
Xu, D.; Li, Z.; Guo, M.; Jiang, B.; Zhang, E.; Zheng, X. Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract. Preprints 2022, 2022010405. https://doi.org/10.20944/preprints202201.0405.v1
Xu, D.; Li, Z.; Guo, M.; Jiang, B.; Zhang, E.; Zheng, X. Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract. Preprints2022, 2022010405. https://doi.org/10.20944/preprints202201.0405.v1
APA Style
Xu, D., Li, Z., Guo, M., Jiang, B., Zhang, E., & Zheng, X. (2022). Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract. Preprints. https://doi.org/10.20944/preprints202201.0405.v1
Chicago/Turabian Style
Xu, D., En Zhang and Xinchuan Zheng. 2022 "Quantitative Determination and Fingerprint of Phenolic Compounds with Anti-inflammatory Activities for the Quality Control of Mangosteen Extract" Preprints. https://doi.org/10.20944/preprints202201.0405.v1
Abstract
The extracts of Garcinia mangostana L. (mangosteen) are traditionally used for medicinal purposes in Asia. Phenols and xanthones are the two main phenolic compounds with anti-inflammatory activities in the pericarps of mangosteen. A simple and economic reverse-phase high-performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous quantification of phenols (catechin, epicatechin, and procyanidin B2) in mangosteen. The mobile phase was acetonitrile: water (12:88) at 30°C, the flow rate was 1 mL/min, and the detection wavelength was 230 nm. The results showed good linear relationships for catechin from 50-250 ng and for epicatechin and procyanidin B2 from 250-1250 ng. The RP-HPLC fingerprint determination method of total xanthones in mangosteen was also established to provide a new method for quality control of mangosteen extract. The mobile phase was a methanol-water gradient elution at 30°C, the flow rate was 1 mL/min, and the detection wavelength was 320 nm. The similarity of fingerprints of 10 batches of total xanthones was more than 0.90. These methods had good precision, reproducibility, and stability and can be applied in the quality control of mangosteen extracts for medicinal purposes.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
