Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

PirAB from Vibrio parahaemolyticus Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in Penaeus vannamei

Version 1 : Received: 11 January 2022 / Approved: 14 January 2022 / Online: 14 January 2022 (11:12:55 CET)

How to cite: Soto-Rodriguez, S.A.; Lozano-Olvera, R.; Ramos-Clamont Montfort, G.; Zenteno, E.; Sánchez-Salgado, J.L.; Vibanco-Pérez, N.; Rendón Aguilar, K.G. PirAB from Vibrio parahaemolyticus Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in Penaeus vannamei. Preprints 2022, 2022010200 (doi: 10.20944/preprints202201.0200.v1). Soto-Rodriguez, S.A.; Lozano-Olvera, R.; Ramos-Clamont Montfort, G.; Zenteno, E.; Sánchez-Salgado, J.L.; Vibanco-Pérez, N.; Rendón Aguilar, K.G. PirAB from Vibrio parahaemolyticus Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in Penaeus vannamei. Preprints 2022, 2022010200 (doi: 10.20944/preprints202201.0200.v1).

Abstract

PirAB is a binary protein complex secreted by specific strains of Vibrio parahaemolyticus (Vp) that harbor the pVA1 virulence plasmid and express PirAVp and PirBVp toxins. PirABVp causes acute hepatopancreatic necrosis disease (AHPND), a newly emergent penaeid shrimp disease that can cause 70–100% mortality and has resulted in great economic losses since its appearance. The cy-totoxic effect of PirABVp on the epithelial cell of the shrimp hepatopancreas has been extensively reported. Our studies found that the PirBVp subunit has lectin activity and recognizes mucin-like O-glycosidic structures in the shrimp hepatopancreas. The PirAVp subunit may have a stabiliza-tion function of the binary complex. However, we also found that Vp AHPND changes the water microbiota community structure and causes a significant reduction in several bacteria, especially Neptuniibacter spp. We propose that the PirABvp toxin could exhibit a dual role: damage the shrimp hepatopancreas and kill surrounding bacteria.

Keywords

PirAB; Vibrio parahaemolyticus; AHPND; shrimp; microbiota change

Subject

BIOLOGY, Agricultural Sciences & Agronomy

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