Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Detection of Novel Biomarkers in Saliva of Rheumatoid Arthritis Model Mice by Proteomics and Pathological Analysis

Version 1 : Received: 27 December 2021 / Approved: 28 December 2021 / Online: 28 December 2021 (14:44:54 CET)

How to cite: Sakaguchi, W.; Saruta, J.; Fuchida, S.; Yamamoto, Y.; To, M.; Shimizu, T.; Yakeishi, M.; Tsukinoki, K. Detection of Novel Biomarkers in Saliva of Rheumatoid Arthritis Model Mice by Proteomics and Pathological Analysis. Preprints 2021, 2021120453 (doi: 10.20944/preprints202112.0453.v1). Sakaguchi, W.; Saruta, J.; Fuchida, S.; Yamamoto, Y.; To, M.; Shimizu, T.; Yakeishi, M.; Tsukinoki, K. Detection of Novel Biomarkers in Saliva of Rheumatoid Arthritis Model Mice by Proteomics and Pathological Analysis. Preprints 2021, 2021120453 (doi: 10.20944/preprints202112.0453.v1).

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease in which joints are gradually de-stroyed, and early diagnosis and treatment before joint deformation or destruction is im-portant. The detection of novel RA biomarkers in saliva may facilitate the early detection of RA before the onset of disease. In this study, we conducted a comprehensive proteomic analysis of salivary proteins from RA model mice. Proteins were identified by Western blotting and enzyme-linked immunosorbent assay in serum, saliva, and ankle joints from DBA/1JJmsSlc mice, a model of rheumatoid arthritis. Ankle joints and submandibular glands were hematoxylin and eosin stained and immunostained, and the results were compared with those of control mice. Citrullinated α1 antitrypsin (A1AT, 46 kDa) was commonly detected in saliva, serum, and ankle joints of severe RA model mice, and was confirmed by proteome analysis. Western blotting detected a band corresponding to 46 kDa in serum, saliva and ankle joints, and immunostaining of ankle joints with A1AT an-tibody showed a strong positive signal in the synovium. In DBA/1JJmsSlc mice, not only cyclic citrullinated peptide antibodies but also A1AT may be involved in protein citrulli-nation and contribute to the development and severity of RA.

Keywords

anti-citrullinated protein antibody (ACPA); alpha-1-antitrypsin (A1AT); DBA/1JJmsSlc mice Proteome analysis; rheumatoid arthritis (RA)

Subject

MEDICINE & PHARMACOLOGY, Pathology & Pathobiology

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