Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Isolation and Identification of Three Parvovirus Strains in Raccoon Dogs From Liaoning Province, China

Version 1 : Received: 18 December 2021 / Approved: 22 December 2021 / Online: 22 December 2021 (12:52:50 CET)

How to cite: Guo, X.; Li, Z.; Lv, T.; Cao, H.; Du, X.; Li, Z.; Zhao, C. Isolation and Identification of Three Parvovirus Strains in Raccoon Dogs From Liaoning Province, China. Preprints 2021, 2021120368 (doi: 10.20944/preprints202112.0368.v1). Guo, X.; Li, Z.; Lv, T.; Cao, H.; Du, X.; Li, Z.; Zhao, C. Isolation and Identification of Three Parvovirus Strains in Raccoon Dogs From Liaoning Province, China. Preprints 2021, 2021120368 (doi: 10.20944/preprints202112.0368.v1).

Abstract

To understand the epidemiological status of parvovirus (RDPV) in raccoon dogs, intestinal tissues of raccoon dogs in Liaoning Province of China were collected and evaluated. Three strains of raccoon dog parvovirus were successfully isolated from 12 intestinal tissues. Nine samples were positive for RDPV, with a positive rate of 75%. The VP2 and NS1 genes of the viruses were cloned and subjected to sequencing for analysis. The nucleotide sequences of the VP2 gene showed 99.94% similarity to the CPV-2a/Racoon dog/QHD/2/19(MT183665) strain, and the nucleotide sequences of the NS1 gene showed 99.75% similarity to RDPV-DP1 NS1(MF996335) strain. The three isolates belonging to the CPV-2a cluster were further confirmed by amino acid sequence alignment and phylogenetic analysis. Our study enriched the epidemiological data of parvovirus in raccoon dogs in the investigating region, and the results will be helpful for future investigation of the variations and transmission of raccoon dog parvoviruses.

Keywords

Raccoon dog parvovirus; Epidemiology; VP2 gene; NS1 gene; Evolutionary analysis.

Subject

BIOLOGY, Animal Sciences & Zoology

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