Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Characterization of Circulating DENV-2 During Outbreak in Northern Senegal, Rosso 2018

Idrissa Dieng
* ,
Mignane Ndiaye
,
Marie Henriette Ndione
,
Safietou Sankhe
,
Moussa Moïse Diagne
ORCID logo ,
Samba Niang Sagne
,
Aboubacry Gaye
,
Aliou Barry
,
Gamou Fall
ORCID logo ,
Amadou Alpha Sall
,
Cheikh Loucoubar
ORCID logo ,
Oumar Faye
,
Ousmane Faye
Version 1 : Received: 13 December 2021 / Approved: 16 December 2021 / Online: 16 December 2021 (12:06:07 CET)

How to cite: Dieng, I.; Ndiaye, M.; Ndione, M.H.; Sankhe, S.; Diagne, M.M.; Sagne, S.N.; Gaye, A.; Barry, A.; Fall, G.; Sall, A.A.; Loucoubar, C.; Faye, O.; Faye, O. Molecular Characterization of Circulating DENV-2 During Outbreak in Northern Senegal, Rosso 2018. Preprints 2021, 2021120270. https://doi.org/10.20944/preprints202112.0270.v1 Dieng, I.; Ndiaye, M.; Ndione, M.H.; Sankhe, S.; Diagne, M.M.; Sagne, S.N.; Gaye, A.; Barry, A.; Fall, G.; Sall, A.A.; Loucoubar, C.; Faye, O.; Faye, O. Molecular Characterization of Circulating DENV-2 During Outbreak in Northern Senegal, Rosso 2018. Preprints 2021, 2021120270. https://doi.org/10.20944/preprints202112.0270.v1

Abstract

Globally 390 millions of people are at risk of dengue infection; over the past 50 years the virus incidence increased thirty-fold. In Senegal, an unprecedented occurrence of outbreaks and sporadic cases was noticed since 2017. In October 2018 an outbreak of DENV-2 was reported in Rosso area in the north of Senegal at the border with Mauritania. Out of the 187 blood specimen samples collected, 27 were positives by qRT-PCR and 8 were serologically positive for DENV IgM. Serotyping using qRT-PCR reveals that isolates were positive for DENV-2. A subset of DENV-2 positives samples was selected and subjected to full genome sequencing followed by phylogenetic analysis. Analysis of 06 nearly completed genome sequences (n= 6) revealed that isolates belong to the cosmopolitan genotype and are closely related to the Mauritanian strains detected between 2017 and 2018 and those detected in many West African countries such as Burkina Faso or Cote d’Ivoire. Our results suggest a transboundary circulation of the DENV-2 cosmopolitan genotype between Senegal and Mauritania and call for a need of coordinated surveillance of arboviruses between these two countries. Interestingly, high level of homology between West African isolates highlights endemicity and call for a set-up of sub-regional viral genomic surveillance which will lead to a better understanding of viral dynamic, transmission and spread across Africa.

Keywords

Dengue; Dengue virus; cosmopolitan genotype; Senegal; Mauritania; Molecular characterization; Full genome sequencing

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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