Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genomic Analysis of the First European Bacteriophages with Depolymerase Activity and Biocontrol Efficacy Against the Phytopathogen Ralstonia solanacearum

Version 1 : Received: 1 December 2021 / Approved: 3 December 2021 / Online: 3 December 2021 (10:36:47 CET)

A peer-reviewed article of this Preprint also exists.

Biosca, E.G.; Català-Senent, J.F.; Figàs-Segura, À.; Bertolini, E.; López, M.M.; Álvarez, B. Genomic Analysis of the First European Bacteriophages with Depolymerase Activity and Biocontrol Efficacy against the Phytopathogen Ralstonia solanacearum. Viruses 2021, 13, 2539. Biosca, E.G.; Català-Senent, J.F.; Figàs-Segura, À.; Bertolini, E.; López, M.M.; Álvarez, B. Genomic Analysis of the First European Bacteriophages with Depolymerase Activity and Biocontrol Efficacy against the Phytopathogen Ralstonia solanacearum. Viruses 2021, 13, 2539.

Journal reference: Viruses 2021, 13, 2539
DOI: 10.3390/v13122539

Abstract

Ralstonia solanacearum is the causative agent of bacterial wilt, one of the most destructive plant diseases. While chemical control has an environmental impact, biological control strategies can allow sustainable agrosystems. Three lytic bacteriophages (phages) of R. solanacearum with biocontrol capacity in environmental water and plant were isolated from river water in Europe but not fully characterized, their genomic characterization being fundamental to understand their biology. In this work, the phage genomes were sequenced and subjected to bioinformatic analysis. The morphology was also observed by electron microscopy. Phylogenetic analyses were performed with a selection of phages able to infect R. solanacearum and the closely related phytopathogenic species R. pseudosolanacearum. The results indicated that the genomes of vRsoP-WF2, vRsoP-WM2 and vRsoP-WR2 range from 40,688 to 41,158 bp with almost 59% GC-contents, 52 ORFs in vRsoP-WF2 and vRsoP-WM2, and 53 in vRsoP-WR2 but, with only 22 or 23 predicted proteins with functional homologs in databases. Among them, two lysins and one exopolysaccharide (EPS) depolymerase, this type of depolymerase being identified in R. solanacearum phages for the first time. These three European phages belong to the same novel species within the Gyeongsanvirus, Autographiviridae family (formerly Podoviridae). These genomic data will contribute to a better understanding of the abilities of these phages to damage host cells and, consequently, to an improvement in the biological control of R. solanacearum.

Keywords

bacterial wilt; biological control; phage; microscopy; sequencing; molecular characterization; genomic characterization; depolymerase

Subject

BIOLOGY, Agricultural Sciences & Agronomy

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.