Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Delineation of the Ancestral Tus-Dependent Replication Fork Trap

Casey Toft
ORCID logo ,
Morgane Moreau
,
Jiri Perutka
,
Savitri Mandapati
,
Peter Enyeart
,
Alanna Sorenson
ORCID logo ,
Andrew D. Ellington
,
Patrick Schaeffer
*
Version 1 : Received: 24 November 2021 / Approved: 29 November 2021 / Online: 29 November 2021 (12:52:31 CET)

A peer-reviewed article of this Preprint also exists.

Toft, C.J.; Moreau, M.J.J.; Perutka, J.; Mandapati, S.; Enyeart, P.; Sorenson, A.E.; Ellington, A.D.; Schaeffer, P.M. Delineation of the Ancestral Tus-Dependent Replication Fork Trap. Int. J. Mol. Sci. 2021, 22, 13533. Toft, C.J.; Moreau, M.J.J.; Perutka, J.; Mandapati, S.; Enyeart, P.; Sorenson, A.E.; Ellington, A.D.; Schaeffer, P.M. Delineation of the Ancestral Tus-Dependent Replication Fork Trap. Int. J. Mol. Sci. 2021, 22, 13533.

Abstract

In Escherichia coli, DNA replication termination is orchestrated by two clusters of Ter sites forming a DNA replication fork trap when bound by Tus proteins. The formation of a ‘locked’ Tus-Ter complex is essential for halting incoming DNA replication forks. However, the absence of replication fork arrest at some Ter sites raised questions about their significance. In this study, we examined the genome-wide distribution of Tus and found that only the six innermost Ter sites (TerA-E and G) were significantly bound by Tus. We also found that a single ectopic insertion of TerB in its non-permissive orientation could not be achieved, advocating against a need for ‘back-up’ Ter sites. Finally, examination of the genomes of a variety of Enterobacterales revealed a new replication fork trap architecture mostly found outside the Enterobacteriaceae family. Taken together, our data enabled the delineation of a narrow ancestral Tus-dependent DNA replication fork trap consisting of only two Ter sites.

Keywords

Replication fork trap; Tus-Ter; dif; ChIP-Seq; GC-skew; Enterobacterales

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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