Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids

Gency Gunasingh; Alexander Browning; Nikolas Haass

doi:10.20944/preprints202111.0488.v2

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preprints.org > biology and life sciences > immunology and microbiology > doi: 10.20944/preprints202111.0488.v2

Preprint Technical Note Version 2 Preserved in Portico This version is not peer-reviewed

Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids

Gency Gunasingh

^* ,

Alexander Browning

^* ,

Nikolas Haass

Version 1 : Received: 23 November 2021 / Approved: 25 November 2021 / Online: 25 November 2021 (16:15:34 CET)
Version 2 : Received: 30 March 2022 / Approved: 31 March 2022 / Online: 31 March 2022 (09:57:33 CEST)

How to cite: Gunasingh, G.; Browning, A.; Haass, N. Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids. Preprints 2021, 2021110488. https://doi.org/10.20944/preprints202111.0488.v2 Gunasingh, G.; Browning, A.; Haass, N. Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids. Preprints 2021, 2021110488. https://doi.org/10.20944/preprints202111.0488.v2

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MDPI and ACS Style

Gunasingh, G.; Browning, A.; Haass, N. Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids. Preprints 2021, 2021110488. https://doi.org/10.20944/preprints202111.0488.v2

APA Style

Gunasingh, G., Browning, A., & Haass, N. (2022). Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids. Preprints. https://doi.org/10.20944/preprints202111.0488.v2

Chicago/Turabian Style

Gunasingh, G., Alexander Browning and Nikolas Haass. 2022 "Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids" Preprints. https://doi.org/10.20944/preprints202111.0488.v2

Abstract

Tumour spheroids are fast becoming commonplace in basic cancer research and drug development. Obtaining high-quality data relating to the inner structure of spheroids is important for analysis, yet existing techniques often use equipment that is not commonly available, are expensive, laborious, cause significant size distortion, or are limited to relatively small spheroids. We present a high-throughput method of mounting, clearing, and imaging tumour spheroids that causes minimal size distortion. Spheroids are mounted in an agarose gel to prevent movement, cleared using a solution prepared from commonly available materials, and imaged using confocal microscopy. We find that our method yields high quality two- and three-dimensional images that provide information about the inner structure of spheroids.

Supplementary and Associated Material

https://github.com/ap-browning/SpheroidMounting: Code and data

Keywords

spheroid; tumour spheroid; mounting; clearing; microscopy; structure; confocal

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Comments (1)

Comment 1

Received: 31 March 2022
Commenter: Alexander Browning
Commenter's Conflict of Interests: Author

Comment: Added Fig 3. Seperated results and discussion section.

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