Version 1
: Received: 18 November 2021 / Approved: 19 November 2021 / Online: 19 November 2021 (13:02:18 CET)
How to cite:
Santos, K.R.; Souza, F.N.; Batista, C.F.; da Silva, G.G.; Rennó, F.P.; Heinemann, M.B.; Della Libera, A.M.M.P. Expression of L-selectin (CD62L) and CD44 on Bovine Lymphocytes and WC1.1+ γδ T Cells under Stimulation with Staphylococcus aureus. Preprints2021, 2021110354. https://doi.org/10.20944/preprints202111.0354.v1
Santos, K.R.; Souza, F.N.; Batista, C.F.; da Silva, G.G.; Rennó, F.P.; Heinemann, M.B.; Della Libera, A.M.M.P. Expression of L-selectin (CD62L) and CD44 on Bovine Lymphocytes and WC1.1+ γδ T Cells under Stimulation with Staphylococcus aureus. Preprints 2021, 2021110354. https://doi.org/10.20944/preprints202111.0354.v1
Santos, K.R.; Souza, F.N.; Batista, C.F.; da Silva, G.G.; Rennó, F.P.; Heinemann, M.B.; Della Libera, A.M.M.P. Expression of L-selectin (CD62L) and CD44 on Bovine Lymphocytes and WC1.1+ γδ T Cells under Stimulation with Staphylococcus aureus. Preprints2021, 2021110354. https://doi.org/10.20944/preprints202111.0354.v1
APA Style
Santos, K.R., Souza, F.N., Batista, C.F., da Silva, G.G., Rennó, F.P., Heinemann, M.B., & Della Libera, A.M.M.P. (2021). Expression of L-selectin (CD62L) and CD44 on Bovine Lymphocytes and WC1.1+ γδ T Cells under Stimulation with Staphylococcus aureus. Preprints. https://doi.org/10.20944/preprints202111.0354.v1
Chicago/Turabian Style
Santos, K.R., Marcos B. Heinemann and Alice M. M. P. Della Libera. 2021 "Expression of L-selectin (CD62L) and CD44 on Bovine Lymphocytes and WC1.1+ γδ T Cells under Stimulation with Staphylococcus aureus" Preprints. https://doi.org/10.20944/preprints202111.0354.v1
Abstract
The present study explored the expression of CD62L and CD44 by bovine peripheral blood mononuclear cells (PBMCs) and WC1.1+ γδ T cells under Staphylococcus aureus cell culture stimu-lation. In this study, peripheral blood cells were isolated from ten dairy cows and cocultured with S. aureus. Afterward, the γδ T cell subpopulation and the expression of CD44, CD62L and prolif-erative (Ki67+) cells were evaluated by flow cytometry. Our results showed that the percentages of proliferative PBMCs and WC1.1+ γδ T cells were higher when stimulated with S. aureus. The percentage of CD44+ cells increased in S. aureus-stimulated cultured PMBCs and WC1.1+ γδ T cells, as did the CD44 geometric mean fluorescence intensity (GMFI). The rate of CD62L cells did not differ among groups for either PBMCs or WC1.1+ γδ T cells. A higher GMFI of CD62L in prolif-erative PBMCs than nonproliferative PBMCs upon stimulation with S. aureus was detected, whereas no impact on the GMFI of CD62L was observed in WC1.1+ cells. In summary, our study identified that S. aureus was associated with high expression of CD44 in overall PBMCs and WC1.1+ γδ T cells, and they could generate memory WC1.1+ γδ T cells, preferably central memory cells.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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