Version 1
: Received: 17 November 2021 / Approved: 18 November 2021 / Online: 18 November 2021 (14:41:02 CET)
How to cite:
Liu, Y. A Peptidoform Matching Strategy in Bottom-Up Proteomics for Studying Functions of Post-Translational Modifications. Preprints2021, 2021110333. https://doi.org/10.20944/preprints202111.0333.v1
Liu, Y. A Peptidoform Matching Strategy in Bottom-Up Proteomics for Studying Functions of Post-Translational Modifications. Preprints 2021, 2021110333. https://doi.org/10.20944/preprints202111.0333.v1
Liu, Y. A Peptidoform Matching Strategy in Bottom-Up Proteomics for Studying Functions of Post-Translational Modifications. Preprints2021, 2021110333. https://doi.org/10.20944/preprints202111.0333.v1
APA Style
Liu, Y. (2021). A Peptidoform Matching Strategy in Bottom-Up Proteomics for Studying Functions of Post-Translational Modifications. Preprints. https://doi.org/10.20944/preprints202111.0333.v1
Chicago/Turabian Style
Liu, Y. 2021 "A Peptidoform Matching Strategy in Bottom-Up Proteomics for Studying Functions of Post-Translational Modifications" Preprints. https://doi.org/10.20944/preprints202111.0333.v1
Abstract
Protein translational modifications (PTMs) generate an enormous, but as yet undetermined, expansion of the expressed proteoforms. In this Viewpoint, we firstly differentiate the concepts of proteoform and peptidoform by reviewing and discussing previous literature. We show that the current PTM biological investigation and annotation largely follow a PTM site-specific rather than proteoform-specific approach. We further illustrate a potentially useful matching strategy in which a particular “modified peptidoform” is matched to the corresponding “unmodified peptidoform” as a reference for the quantitative analysis between samples and conditions. We suggest this strategy could provide directly relevant information for learning the PTM site-specific biological functions. Accordingly, we advocate for the wider use of the nomenclature “peptidoform” in the future bottom-up proteomic studies.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
