Version 1
: Received: 13 November 2021 / Approved: 15 November 2021 / Online: 15 November 2021 (19:24:40 CET)
How to cite:
Yoon, J.S.J.; Baksh, A.; Chamberlain, T.C.; Mui, A.L. The Strand Specific Regulation of miR-155-3p in Response to Lipopolysaccharide and Interleukin-10 Stimulation Requires FUBP1 Protein. Preprints2021, 2021110272. https://doi.org/10.20944/preprints202111.0272.v1.
Yoon, J.S.J.; Baksh, A.; Chamberlain, T.C.; Mui, A.L. The Strand Specific Regulation of miR-155-3p in Response to Lipopolysaccharide and Interleukin-10 Stimulation Requires FUBP1 Protein. Preprints 2021, 2021110272. https://doi.org/10.20944/preprints202111.0272.v1.
Cite as:
Yoon, J.S.J.; Baksh, A.; Chamberlain, T.C.; Mui, A.L. The Strand Specific Regulation of miR-155-3p in Response to Lipopolysaccharide and Interleukin-10 Stimulation Requires FUBP1 Protein. Preprints2021, 2021110272. https://doi.org/10.20944/preprints202111.0272.v1.
Yoon, J.S.J.; Baksh, A.; Chamberlain, T.C.; Mui, A.L. The Strand Specific Regulation of miR-155-3p in Response to Lipopolysaccharide and Interleukin-10 Stimulation Requires FUBP1 Protein. Preprints 2021, 2021110272. https://doi.org/10.20944/preprints202111.0272.v1.
Abstract
MicroRNA-155 (miR-155) expression promotes inflammatory responses in macrophages. Activation of macrophages with lipopolysaccharide (LPS) elevates miR-155, while the anti-inflammatory cytokine interleukin-10 (IL10) reduces miR-155 levels. MiR-155 exists in two forms, miR-155-5p and miR-155-3p, produced from the precursor of miR-155 (pre-miR-155). MiR-155-5p is the most abundant strand in activated macrophages, but in response to LPS, the miR-155-3p level is upregulated first, followed by miR-155-5p later. We have previously identified CELF2 protein which interacts with pre-miR-155 and impairs miR-155-5p expression. We now show that CELF2 only regulates the miR-155-5p expression and that another protein FUBP1 controls miR-155-3p levels in response to LPS and IL10.
Keywords
miR-155; FUBP1; strand selection; interleukin-10
Subject
LIFE SCIENCES, Biochemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
