Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System

Version 1 : Received: 10 November 2021 / Approved: 11 November 2021 / Online: 11 November 2021 (12:57:06 CET)

How to cite: Wang, S.; Tang, Y.; Wei, J.; Wang, S.; Peng, G.; Li, Y.; Wu, S. Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System. Preprints 2021, 2021110210 (doi: 10.20944/preprints202111.0210.v1). Wang, S.; Tang, Y.; Wei, J.; Wang, S.; Peng, G.; Li, Y.; Wu, S. Detection of Klebsiella pneumoniae DNA by PCR-Based CRISPR-lbCas12a System. Preprints 2021, 2021110210 (doi: 10.20944/preprints202111.0210.v1).

Abstract

Klebsiella pneumonia (K. pneumoniae) is a Gram-negative bacterium that causes nosocomial infections in the lung, bloodstream, and urinary tract. Therefore, detecting K. pneumoniae in early time is important in preventing severe infections. However, clinical detection of K. pneumoniae requires a long time of agar plate culture. Nucleic acid detection like qPCR is precise but requires expensive equipment. Recent research reveals that collateral cleavage activity of CRISPR-LbCas12a has been applied in nucleic acid detection. In this study, PCR combined with CRISPR-LbCas12a targeting the K. pneumoniae system was established. This system showed excellent detection specificity and sensitivity in both bench work and clinical samples. Due to its advantages, its application can meet different detection requirements in health centers where qPCR is not accessible.

Keywords

CRISPR-Cas, Nucleic acid detection, Klebsiella pneumonia

Subject

MEDICINE & PHARMACOLOGY, Pathology & Pathobiology

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