Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1

Version 1 : Received: 18 October 2021 / Approved: 19 October 2021 / Online: 19 October 2021 (10:29:01 CEST)

A peer-reviewed article of this Preprint also exists.

Mülner, P.; Schwarz, E.; Dietel, K.; Herfort, S.; Jähne, J.; Lasch, P.; Cernava, T.; Berg, G.; Vater, J. Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1. Pathogens 2021, 10, 1485. Mülner, P.; Schwarz, E.; Dietel, K.; Herfort, S.; Jähne, J.; Lasch, P.; Cernava, T.; Berg, G.; Vater, J. Fusaricidins, Polymyxins and Volatiles Produced by Paenibacillus polymyxa Strains DSM 32871 and M1. Pathogens 2021, 10, 1485.

Abstract

Paenibacilli are efficient producers of potent agents against bacterial and fungal pathogens, which are of great interest both for therapeutic applications in medicine as well as in agrobiotechnol-ogy. Lipopeptides produced by such organisms play a major role in their inactivation potential. In this work we investigated two lipopeptide complexes, the fusaricidins and the polymyxins, produced by Paenibacillus polymyxa strains DSM 32871 and M1 by MALDI-TOF mass spectrometry. The fusaricidins show potent antifungal activities and are distinguished by an unusual variabil-ity. For strain DSM 32871 we identified numerous yet unknown variants mass spectrometrically. DSM 32871 produces polymyxins of type E (colistins), while M1 forms polymyxins P. For both strains novel, but not yet completely characterized polymyxin species were detected, which pos-sibly are glycosylated. These compounds may be of interest therapeutically, because polymyxins attain increasing attention as last-resort antibiotics against multiresistant pathogenic Gram-negative bacteria. In addition, the volatilomes of DSM 32781 and M1 were investigated with a GC-MS approach using different cultivation media. Production of volatile organic com-pounds (VOCs) was strain and medium dependent. In particular, strain M1 manifested as an effi-cient VOC-producer that exhibited formation of 25 volatiles in total. A characteristic feature of Paenibacilli is the formation of volatile pyrazine derivatives.

Keywords

P. polymyxa; bioactive peptides; volatiles; MALDI-TOF mass spectrometry; GC-MS

Subject

Biology and Life Sciences, Immunology and Microbiology

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