Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Engineering of Synthetic Transcriptional Switches in Yeast

Version 1 : Received: 1 October 2021 / Approved: 5 October 2021 / Online: 5 October 2021 (11:19:46 CEST)

A peer-reviewed article of this Preprint also exists.

Journal reference: Life 2022, 12, 557
DOI: 10.3390/life12040557


Genetic switches can be utilized for many purposes in synthetic biology including the assembly of complex genetic circuits to achieve sophisticated cellular systems and the construction of biosensors for real-time monitoring of intracellular metabolite concentrations. Although genetic switches have mainly been developed in prokaryotes to date, eukaryotic genetic switches are increasingly being reported as both rational and irrational engineering technologies mature. In this review, we describe genetic switches in yeast based on synthetic transcription factors and/or synthetic promoters. We also discuss directed evolution technologies for the rapid and robust construction of yeast genetic switches.


genetic switch; yeast; synthetic transcription factor; synthetic promoter; directed evolution


LIFE SCIENCES, Biotechnology

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