Tominaga, M.; Kondo, A.; Ishii, J. Engineering of Synthetic Transcriptional Switches in Yeast. Life 2022, 12, 557, doi:10.3390/life12040557.
Tominaga, M.; Kondo, A.; Ishii, J. Engineering of Synthetic Transcriptional Switches in Yeast. Life 2022, 12, 557, doi:10.3390/life12040557.
Tominaga, M.; Kondo, A.; Ishii, J. Engineering of Synthetic Transcriptional Switches in Yeast. Life 2022, 12, 557, doi:10.3390/life12040557.
Tominaga, M.; Kondo, A.; Ishii, J. Engineering of Synthetic Transcriptional Switches in Yeast. Life 2022, 12, 557, doi:10.3390/life12040557.
Abstract
Genetic switches can be utilized for many purposes in synthetic biology including the assembly of complex genetic circuits to achieve sophisticated cellular systems and the construction of biosensors for real-time monitoring of intracellular metabolite concentrations. Although genetic switches have mainly been developed in prokaryotes to date, eukaryotic genetic switches are increasingly being reported as both rational and irrational engineering technologies mature. In this review, we describe genetic switches in yeast based on synthetic transcription factors and/or synthetic promoters. We also discuss directed evolution technologies for the rapid and robust construction of yeast genetic switches.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
