Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Grapevine Virology in the Third-Generation Sequencing Era: From Virus Detection to Viral Epitranscriptomics

Version 1 : Received: 16 September 2021 / Approved: 20 September 2021 / Online: 20 September 2021 (10:43:01 CEST)

A peer-reviewed article of this Preprint also exists.

Javaran, V.J.; Moffett, P.; Lemoyne, P.; Xu, D.; Adkar-Purushothama, C.R.; Fall, M.L. Grapevine Virology in the Third-Generation Sequencing Era: From Virus Detection to Viral Epitranscriptomics. Plants 2021, 10, 2355. Javaran, V.J.; Moffett, P.; Lemoyne, P.; Xu, D.; Adkar-Purushothama, C.R.; Fall, M.L. Grapevine Virology in the Third-Generation Sequencing Era: From Virus Detection to Viral Epitranscriptomics. Plants 2021, 10, 2355.

Abstract

Among all economically important plant species in the world, grapevine (Vitis vinifera L.) is the most cultivated fruit plant. It has a significant impact on the economies of many countries through wine and fresh and dried fruit production. In recent years, the grape and wine industry has been facing outbreaks of known and emerging viral diseases across the world. Although high-throughput sequencing (HTS) has been used extensively in grapevine virology, the application and potential of third-generation sequencing have not been explored in understanding grapevine viruses and their impact on the grapevine. Nanopore sequencing, a third-generation technology, can be used for direct sequencing of both RNA and DNA with minimal infrastructure. Compared to other HTS methods, the MinION nanopore platform is faster and more cost-effective and allows for long-read sequencing. Due to the size of the MinION device, it can be easily carried for field viral disease surveillance. This review article discusses grapevine viruses and their diagnostic methods, the principle of nanopore sequencing technology and its application in grapevine virus detection, virus–plant interactions, as well as the characterization of viral RNA modifications.

Keywords

Grapevine; Viral Disease; Diagnostic Methods; RNA Sequencing; Nanopore Sequencing Technology; RNA modifications

Subject

Biology and Life Sciences, Virology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.