Version 1
: Received: 11 September 2021 / Approved: 14 September 2021 / Online: 14 September 2021 (10:02:54 CEST)
How to cite:
Akimoto, J.; Fukami, S.; Ichikawa, M.; nagai, K.; Kohno, M. Rapid Intraoperative Detection of Residual Glioma Cell in Resection Cavity Walls Using a Compact Fluorescence Microscope. Preprints2021, 2021090229. https://doi.org/10.20944/preprints202109.0229.v1
Akimoto, J.; Fukami, S.; Ichikawa, M.; nagai, K.; Kohno, M. Rapid Intraoperative Detection of Residual Glioma Cell in Resection Cavity Walls Using a Compact Fluorescence Microscope. Preprints 2021, 2021090229. https://doi.org/10.20944/preprints202109.0229.v1
Akimoto, J.; Fukami, S.; Ichikawa, M.; nagai, K.; Kohno, M. Rapid Intraoperative Detection of Residual Glioma Cell in Resection Cavity Walls Using a Compact Fluorescence Microscope. Preprints2021, 2021090229. https://doi.org/10.20944/preprints202109.0229.v1
APA Style
Akimoto, J., Fukami, S., Ichikawa, M., nagai, K., & Kohno, M. (2021). Rapid Intraoperative Detection of Residual Glioma Cell in Resection Cavity Walls Using a Compact Fluorescence Microscope. Preprints. https://doi.org/10.20944/preprints202109.0229.v1
Chicago/Turabian Style
Akimoto, J., Kenta nagai and Michihiro Kohno. 2021 "Rapid Intraoperative Detection of Residual Glioma Cell in Resection Cavity Walls Using a Compact Fluorescence Microscope" Preprints. https://doi.org/10.20944/preprints202109.0229.v1
Abstract
Objective: Surgical eradication of malignant glioma cells is theoretically impossible. Therefore, reducing the number of remaining tumor cells around the brain-tumor interface (BTI) is crucial for achieving satisfactory clinical results. The usefulness of fluorescence-guided resection for the treatment of malignant glioma was recently reported, but the detection of infiltrating tumor cells in the BTI using a surgical microscope is not realistic. Therefore, we developed an intraoperative rapid fluorescence cytology system, and evaluated its clinical feasibility for the management of malignant glioma. Materials and methods: Twenty-five selected patients with malignant glioma (newly diagnosed: 17; recurrent: 8) underwent surgical resection under photodiagnosis using photosensitizer Talaporfin sodium and a semiconductor laser. Intraoperatively, a crush smear preparation was made from a tiny amount of tumor tissue, and the fluorescence emitted upon 620/660 nm excitation was evaluated rapidly using a compact fluorescence microscope in the operating theater. Results: Fluorescence intensities of tumor tissues measured using a surgical microscope correlated with the tumor cell densities of tissues evaluated by measuring the red fluorescence emitted from the cytoplasm of tumor cells using a fluorescence microscope. A “weak fluorescence” indicated a reduction in the tumor cell density, whereas “no fluorescence” did not indicate the complete eradication of the tumor cells, but indicated that few tumor cells were emitting fluorescence.Conclusion: The rapid intraoperative detection of fluorescence from glioma cells using a compact fluorescence microscope was a useful to evaluate the presence of tumor cells in the resection cavity walls, and provides surgical implications for the more complete resection of malignant gliomas.
Medicine and Pharmacology, Oncology and Oncogenics
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
