Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured Under Different Conditions

Version 1 : Received: 20 August 2021 / Approved: 23 August 2021 / Online: 23 August 2021 (13:31:33 CEST)

How to cite: Hvid, K.G.; Petitjean, I.I.; Barooji, Y.F.; Brickman, J.M.; Oddershede, L.B.; Bendix, P.M. Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured Under Different Conditions. Preprints 2021, 2021080444 (doi: 10.20944/preprints202108.0444.v1). Hvid, K.G.; Petitjean, I.I.; Barooji, Y.F.; Brickman, J.M.; Oddershede, L.B.; Bendix, P.M. Changes in Cell Morphology and Actin Organization in Embryonic Stem Cells Cultured Under Different Conditions. Preprints 2021, 2021080444 (doi: 10.20944/preprints202108.0444.v1).

Abstract

The cellular cytoskeleton provides the cell with a mechanical rigidity which allows mechanical interaction between cells and the extracellular environment. The actin structure plays a key role in mechanical events like motility, or establishment of cell polarity. From the earliest stages of development, as represented by ex vivo expansion of naïve embryonic stem cells (ESCs), the critical mechanical role of the actin structure is becoming recognized as a vital cue for correct segregation and lineage control of cells and as a regulatory structure that controls several transcription factors. Naïve ESCs have a characteristic morphology and the ultrastructure that underlies this condition remains to be further investigated. Here, we investigate the 3D actin cytoskeleton of naïve mouse ESCs using super resolution optical reconstruction microscopy (STORM). We investigate the morphological, cytoskeletal and mechanical changes in cells cultured in 2i or Serum/LIF media reflecting a homogenous preimplantation cell state and a state that is closer to embarking on differentiation. STORM imaging showed that the peripheral actin structure undergoes a dramatic change between the two media conditions. We also detected micro-rheological differences in the cell periphery between the cells cultured in these two media correlating well with the observed nano-architecture of the ESCs in the two different culture conditions. These results pave the way for linking physical properties and cytoskeletal architecture to cell morphology during early development.

Keywords

actin cytoskeleton; super-resolution microscopy; embryonic stem cells; primed embryonic stem cells; micro-rheology; cell culturing; optical tweezers

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.