Working Paper Article Version 1 This version is not peer-reviewed

Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using Punica granatum Leaves

Version 1 : Received: 11 August 2021 / Approved: 11 August 2021 / Online: 11 August 2021 (11:40:07 CEST)

How to cite: Singhal, M.; Chatterjee, S.; Kumar, A.; Syed, A.; Bahkali, A.H.; Gupta, N.; Nimesh, S. Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using Punica granatum Leaves. Preprints 2021, 2021080257 Singhal, M.; Chatterjee, S.; Kumar, A.; Syed, A.; Bahkali, A.H.; Gupta, N.; Nimesh, S. Exploring the Antibacterial and Antibiofilm Efficacy of Silver Nanoparticles Biosynthesized Using Punica granatum Leaves. Preprints 2021, 2021080257

Abstract

The current research work illustrates an economic and rapid approach towards biogenic synthesis of silver nanoparticles using aqueous Punica granatum leaves extract (PGL-AgNPs). The optimization of major parameters involved in the biosynthesis process was done using Box-Behnken Design (BBD). The effects of different independent variables (parameters) namely concentration of AgNO3, temperature and ratio of extract to AgNO3on response viz. particle size and polydispersity index were analyzed. As a result of experiment designing, 17 reactions were generated which were further validated experimentally. The statistical and mathematical approaches were employed on these reactions in order to interpret the relationship between the factors and responses. The biosynthesized nanoparticles were initially characterized by UV-vis spectrophotometry followed by physicochemical analysis for determination of particle size, polydispersity index and zeta potential via dynamic light scattering (DLS), SEM and EDX studies. Moreover, the determination of functional group present in the leaves extract and PGL-AgNPs was done by FTIR. Antibacterial and antibiofilm efficacies of PGL-AgNPs against Gram-positive and Gram-negative bacteria were further determined. The physicochemical studies suggested that PGL-AgNPs were round in shape and of ~ 37.5 nm in size with uniform distribution. Our studies suggested that PGL-AgNPs exhibit potent antibacterial, and antibiofilm properties.

Keywords

Punica granatum leaves extract; silver nanoparticles; Box-Behnken design; antibacterial; antibiofilm

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