Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Automatic Lab on PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

Version 1 : Received: 9 August 2021 / Approved: 11 August 2021 / Online: 11 August 2021 (11:02:00 CEST)

A peer-reviewed article of this Preprint also exists.

Urbano-Gámez, J.D.; Perdigones, F.; Quero, J.M. Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications. Micromachines 2021, 12, 1071. Urbano-Gámez, J.D.; Perdigones, F.; Quero, J.M. Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications. Micromachines 2021, 12, 1071.

Journal reference: Micromachines 2021, 12, 1071
DOI: 10.3390/mi12091071

Abstract

In this paper, a prototype of an automatic lab on PCB for agarose preparation and electrophoresis is developed. The dimensions of the device are 38×34 mm2 and includes a conductivity sensor for detecting the TAE buffer (Tris-Acetate-EDTA buffer), a microheater for mixing, a NTC thermistor for controlling the temperature, a LDR sensor for measuring the transparency of the mixture, and two electrodes for performing the electrophoresis. The agarose preparation functions are governed by a microcontroller. The device requires a PMMA structure to define the wells of the agarose gel, and to release the electrodes from the agarose. The maximum voltage and current that the system requires are 40 V to perform the electrophoresis, and 1 A for activating the microheater. The chosen temperature for mixing is 80ºC, with a mixing time of 10 min. In addition, the curing time is about 30 min. This device is intended to be integrated as a part of a larger lab on PCB system for DNA amplification and detection. However, it can be used to migrate DNA amplified in conventional thermocyclers. Moreover, the device can be modified for preparing larger agarose gels and performing electrophoresis in an automatic manner.

Keywords

Lab on PCB; Electrophoresis; Biomedical applications; Agarose

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