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Molecular Characterization of a Functional Membrane-Associated Progesterone Receptor Component 2 (PGRMC-2) in Maturing Oocytes of the Human Parasite Nematode Trichinella spiralis: Implications to the Host-Parasite Relationship
Version 1
: Received: 22 July 2021 / Approved: 26 July 2021 / Online: 26 July 2021 (09:08:31 CEST)
How to cite:
Morales-Montor, J.; Colin-Oviedo, Á.; González-González, G.M.; Palma-Nicolás, J.P.; Sánchez-González, A.; Nava-Castro, K.E.; Dominguez-Ramirez, L.; García-Varela, M.; Río-Araiza, V.H.D.; Hernández-Bello, R. Molecular Characterization of a Functional Membrane-Associated Progesterone Receptor Component 2 (PGRMC-2) in Maturing Oocytes of the Human Parasite Nematode Trichinella spiralis: Implications to the Host-Parasite Relationship. Preprints2021, 2021070555. https://doi.org/10.20944/preprints202107.0555.v1
Morales-Montor, J.; Colin-Oviedo, Á.; González-González, G.M.; Palma-Nicolás, J.P.; Sánchez-González, A.; Nava-Castro, K.E.; Dominguez-Ramirez, L.; García-Varela, M.; Río-Araiza, V.H.D.; Hernández-Bello, R. Molecular Characterization of a Functional Membrane-Associated Progesterone Receptor Component 2 (PGRMC-2) in Maturing Oocytes of the Human Parasite Nematode Trichinella spiralis: Implications to the Host-Parasite Relationship. Preprints 2021, 2021070555. https://doi.org/10.20944/preprints202107.0555.v1
Morales-Montor, J.; Colin-Oviedo, Á.; González-González, G.M.; Palma-Nicolás, J.P.; Sánchez-González, A.; Nava-Castro, K.E.; Dominguez-Ramirez, L.; García-Varela, M.; Río-Araiza, V.H.D.; Hernández-Bello, R. Molecular Characterization of a Functional Membrane-Associated Progesterone Receptor Component 2 (PGRMC-2) in Maturing Oocytes of the Human Parasite Nematode Trichinella spiralis: Implications to the Host-Parasite Relationship. Preprints2021, 2021070555. https://doi.org/10.20944/preprints202107.0555.v1
APA Style
Morales-Montor, J., Colin-Oviedo, Á., González-González, G.M., Palma-Nicolás, J.P., Sánchez-González, A., Nava-Castro, K.E., Dominguez-Ramirez, L., García-Varela, M., Río-Araiza, V.H.D., & Hernández-Bello, R. (2021). Molecular Characterization of a Functional Membrane-Associated Progesterone Receptor Component 2 (PGRMC-2) in Maturing Oocytes of the Human Parasite Nematode <i>Trichinella spiralis</i>: Implications to the Host-Parasite Relationship. Preprints. https://doi.org/10.20944/preprints202107.0555.v1
Chicago/Turabian Style
Morales-Montor, J., Víctor Hugo Del Río-Araiza and Romel Hernández-Bello. 2021 "Molecular Characterization of a Functional Membrane-Associated Progesterone Receptor Component 2 (PGRMC-2) in Maturing Oocytes of the Human Parasite Nematode <i>Trichinella spiralis</i>: Implications to the Host-Parasite Relationship" Preprints. https://doi.org/10.20944/preprints202107.0555.v1
Abstract
We explored the hypothesis that progesterone direct effect on Trichinella spiralis might be mediated indeed by a new steroid-binding parasite protein. Our first results showed that Progesterone decreases the parasite molting rate. We amplify, isolated, cloned and sequenced the PGRMC2 sequence using specific primers from known species. Furthermore, we expressed the protein and developed an antibody to performance immunofluorescent confocal microscopy, where detected that parasite cells showed expression of a P4-binding protein exclusively located at the oocyte and the parasite´s cuticle. Presence of the PGRMC2 protein in these cells was also confirmed by western blot and flow cytometry. Molecular modeling studies accompanied by computer docking using the sequenced protein showed that PGRMC2 is potentially able to bind steroid hormones such as progesterone, estradiol, testosterone, and dihydrodrotestosterone with different affinities. Phylogenetic analysis and sequence alignment clearly demonstrated that Trichinella spiralis PGRMC2 is related to a steroid-binding protein of another platyhelminths. Progesterone may probably act upon Trichinella spiralis oocytes probably by binding to PGRMC2. This research has implications in the field of host-parasite co-evolution as well as the sex-associated susceptibility to this infection. In a more practical matter, present results may contribute to the molecular design of new drugs with anti-parasite actions.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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