PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Positive Impact of Fibronectin in Stimulation Human Adipose-derived Mesenchymal Stem Cells Attachment Seeded Into Polytetrafluoroethylene Patch for Future Surgical Closure of Atrial and Ventricular Septal Defect
Version 1
: Received: 22 July 2021 / Approved: 23 July 2021 / Online: 23 July 2021 (12:30:21 CEST)
How to cite:
Suryawan, I.G.R.; Ratri, A.K.; Andrianto, A.; Ardiana, M.; Nugraha, R.A. Positive Impact of Fibronectin in Stimulation Human Adipose-derived Mesenchymal Stem Cells Attachment Seeded Into Polytetrafluoroethylene Patch for Future Surgical Closure of Atrial and Ventricular Septal Defect. Preprints2021, 2021070544. https://doi.org/10.20944/preprints202107.0544.v1
Suryawan, I.G.R.; Ratri, A.K.; Andrianto, A.; Ardiana, M.; Nugraha, R.A. Positive Impact of Fibronectin in Stimulation Human Adipose-derived Mesenchymal Stem Cells Attachment Seeded Into Polytetrafluoroethylene Patch for Future Surgical Closure of Atrial and Ventricular Septal Defect. Preprints 2021, 2021070544. https://doi.org/10.20944/preprints202107.0544.v1
Suryawan, I.G.R.; Ratri, A.K.; Andrianto, A.; Ardiana, M.; Nugraha, R.A. Positive Impact of Fibronectin in Stimulation Human Adipose-derived Mesenchymal Stem Cells Attachment Seeded Into Polytetrafluoroethylene Patch for Future Surgical Closure of Atrial and Ventricular Septal Defect. Preprints2021, 2021070544. https://doi.org/10.20944/preprints202107.0544.v1
APA Style
Suryawan, I.G.R., Ratri, A.K., Andrianto, A., Ardiana, M., & Nugraha, R.A. (2021). Positive Impact of Fibronectin in Stimulation Human Adipose-derived Mesenchymal Stem Cells Attachment Seeded Into Polytetrafluoroethylene Patch for Future Surgical Closure of Atrial and Ventricular Septal Defect. Preprints. https://doi.org/10.20944/preprints202107.0544.v1
Chicago/Turabian Style
Suryawan, I.G.R., Meity Ardiana and Ricardo Adrian Nugraha. 2021 "Positive Impact of Fibronectin in Stimulation Human Adipose-derived Mesenchymal Stem Cells Attachment Seeded Into Polytetrafluoroethylene Patch for Future Surgical Closure of Atrial and Ventricular Septal Defect" Preprints. https://doi.org/10.20944/preprints202107.0544.v1
Abstract
Background. Polytetrafluoroethylene (PTFE) patch is commonly used during surgical closure for atrial septal defect (ASD) and/or ventricular septal defect (VSD). It has several limitations such as inability to grow, repair, and remodel. Aneurysm formation, thrombosis, and the inability of patches to grow or remodel are usual, especially in children and young adults. To tackle these limitations, we try to use fibronectin and human adipose-derived mesenchymal stem cells (hAMSCs) in PTFE patch.Objective. To understanding positive impact of fibronectin to enhance hAMSCs cell-to-cell adherence and cell-to-patch surface attachment into PTFE patch for future ASD or VSD closure. Methods. Cultured of hAMSCs cells were fixated with 15 mL methanol and CD90+, CD105+, CD45- antibodies were labeled FITC, rinsed with PBS and analyzed under fluorescence microscope for 15 minutes. Fibronectin solution 0.1% were used to soak patch scaffolds for approximately 2 hours duration, and then dried for 20 minutes for treatment group. As for control group, Fibronectin solution was not added on the culture. The samples were examined with scanning electron microscope (SEM). Results. SEM examination showed incomplete attachment of the cells even after 10 days on control group at 1.14 ±1.13 (Figure 2). In contrast, treatment group showed more cells attached to the patch surface at 31.25 ±13.28 (p 0.000) (Figure 3). Observation at 5 days was 17.67 ± 20.21, at 7 days was 12.11 ± 10.94, at 10 days was 18.83 ± 23.25. No significant statistical difference of mean cell per view among each treatment group (p 0.802). Conclusion. Fibronectin has a positive impact on hAMSCs attachment seeded onto PTFE patch. These properties, in combination with their developmental plasticity, have generated tremendous interest because of the potential use of hAMSCs in regenerative medicine to replace damaged tissues.
Keywords
ASD; fibronectin; hAMSCs; PTFE patch; VSD
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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