Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Optical Coherence Tomography and Raman Spectroscopy for Sensing of the Bone Demineralization Process

Version 1 : Received: 15 July 2021 / Approved: 16 July 2021 / Online: 16 July 2021 (09:25:51 CEST)

How to cite: Głowacki, M.; Kamińska, A.; Gnyba, M.; Pluciński, J.; Strąkowski, M.R. The Optical Coherence Tomography and Raman Spectroscopy for Sensing of the Bone Demineralization Process. Preprints 2021, 2021070366 (doi: 10.20944/preprints202107.0366.v1). Głowacki, M.; Kamińska, A.; Gnyba, M.; Pluciński, J.; Strąkowski, M.R. The Optical Coherence Tomography and Raman Spectroscopy for Sensing of the Bone Demineralization Process. Preprints 2021, 2021070366 (doi: 10.20944/preprints202107.0366.v1).

Abstract

Aim of the presented research was to develop an optical sensing system to investigate the demineralization process of the bones. Optical measurement techniques are widely used and increasingly adapted in biological and biomedical applications due to their non-destructive nature and safety. Optical examination of the bone condition could facilitate clinical trials and improve the safety of patients. The authors used a set of complementary methods: polarization-sensitive optical coherence tomography (PS-OCT) and Raman spectroscopy. To stimulate the process of demineralization and gradual removal of the hydroxyapatite, the test samples of chicken bones were placed into 10% acetic acid. Measurements were carried out in two series. The first one took two weeks with data acquired every day. In the second series, the measurements were made during one day at an hourly interval (after 1, 2, 3, 5, 7, 10, and 24 hours). Raman spectroscopy was used to evaluate the disappearance of the hydroxyapatite. The relation between the content of hydroxyapatite and images recorded using OCT was analyzed and discussed. Moreover, the polarization properties of the bones have been evaluated. Based on OCT images, the retardation angles of the bones have been calculated. This work presents a preliminary study on the mechanism of bone demineralization and confirms the potential of the applied optical methods.

Subject Areas

bones; polarization-sensitive optical coherence tomography; Raman spectroscopy

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