Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Astrocyte Stimulates Microglial Proliferation and M2 Polarization in vitro through Cross-Talk between Astrocyte and Microglia

Version 1 : Received: 10 July 2021 / Approved: 14 July 2021 / Online: 14 July 2021 (12:42:17 CEST)

How to cite: Kim, S.; Son, Y. Astrocyte Stimulates Microglial Proliferation and M2 Polarization in vitro through Cross-Talk between Astrocyte and Microglia. Preprints 2021, 2021070331 (doi: 10.20944/preprints202107.0331.v1). Kim, S.; Son, Y. Astrocyte Stimulates Microglial Proliferation and M2 Polarization in vitro through Cross-Talk between Astrocyte and Microglia. Preprints 2021, 2021070331 (doi: 10.20944/preprints202107.0331.v1).

Abstract

Microglia are resident immune cells of the central nervous system such as brain-specific macrophages and also known to regulate the innate immune functions of astrocytes through secretory molecules. This conversation plays an important role in brain functions and homeostasis as well as in neuropathologic disease. In this study, we aimed to elucidate whether astrocytes and microglia can cross-talk to induce microglial polarization and proliferation, which can be further regulated under the brain stroke-mimic microenvironment. Microglia in mixed glial culture increased their survival and proliferation and altered to the M2 microglia, whose role was provided by CD11b-GFAP+ astrocytes by showing approximately tenfold increase in microglia cell proliferation after the astrocyte reconstitution. Furthermore, GM-CSF stimulated microglial proliferation approximately tenfold and induced to CCR7+ M1 microglia, whose phenotype could be suppressed by anti-inflammatory cytokines such as IL-4, IL-10, and Substance-P. Also, astrocyte in the microglia co-culture revealed A2 phenotype, which could be activated to A1 astrocyte by TNFα and IFNγ under the stroke-mimic condition. Altogether, astrocyte in the mixed glial culture stimulated the microglia proliferation and M2 polarization possibly through its acquisition of A2 phenotype, both of which could be converted to M1 microglia and A1 astrocytes under the inflammatory stroke-mimic environment. This study demonstrated that microglia and astrocyte can be polarized to M2 microglia and A2 astrocytes respectively through the cross-talk in vitro and provided a system to explore how microglia and astrocyte may behave in the inflammatory disease milieu after in vivo transplantation.

Subject Areas

Microglia; M1/M2 polarization; astrocyte; microglia and astrocyte cross-talk

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.