Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Performance Evaluation for Repair of Hsgc-c5 Carcinoma Cell Using geant4-Dna

Dousatsu Sakata
* ORCID logo ,
Masao Suzuki
,
Ryoichi Hirayama
,
Yasushi Abe
,
Masayuki Muramatsu
,
Shinji Sato
,
Oleg Belov
ORCID logo ,
Ioanna Kyriakou
,
Dimitris Emfietzoglou
,
Susanna Guatelli
,
Sebastien Incerti
ORCID logo ,
Taku Inaniwa
Version 1 : Received: 30 June 2021 / Approved: 1 July 2021 / Online: 1 July 2021 (09:28:54 CEST)

A peer-reviewed article of this Preprint also exists.

Sakata, D.; Suzuki, M.; Hirayama, R.; Abe, Y.; Muramatsu, M.; Sato, S.; Belov, O.; Kyriakou, I.; Emfietzoglou, D.; Guatelli, S.; Incerti, S.; Inaniwa, T. Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA. Cancers 2021, 13, 6046. Sakata, D.; Suzuki, M.; Hirayama, R.; Abe, Y.; Muramatsu, M.; Sato, S.; Belov, O.; Kyriakou, I.; Emfietzoglou, D.; Guatelli, S.; Incerti, S.; Inaniwa, T. Performance Evaluation for Repair of HSGc-C5 Carcinoma Cell Using Geant4-DNA. Cancers 2021, 13, 6046.

Abstract

Track-structure Monte Carlo simulations are useful tools to evaluate initial DNA damage induced by irradiation. In the previous study, we have developed a Gean4-DNA-based application to estimate the cell surviving fraction of V79 cells after irradiation, bridging the gap between the initial DNA damage and the DNA-rejoining kinetics by means of the two-lesion kinetics (TLK) model. However, since the DNA repair performance depends on cell line, the same model parameters cannot be used for the different cell lines. Thus, we extended the Geant4-DNA application with an updated TLK model for the evaluation of DNA damage repair performance in HSGc-C5 carcinoma cells which are typically used for evaluating proton/carbon radiation treatment effects. For this evaluation, we also performed experimental measurements for cell surviving fractions and DNA-rejoining kinetics of the HSGc-C5s cells. Concerning fast- and slow-DNA rejoining, the TLK model parameters were adequately optimized with the simulated initial DNA damage. Using the optimized TLK model, the Geant4-DNA simulation is now able to predict cell survival and DNA-rejoining kinetics for HSGc-C5s cells.

Keywords

Geant4-DNA; DNA repair; Cell surviving fraction

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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