Version 1
: Received: 28 June 2021 / Approved: 29 June 2021 / Online: 29 June 2021 (11:45:54 CEST)
How to cite:
Weber, A.; weber, K.; Pretot, S.; Sillipo, M.; Lombardi, L.; Warfving, N. Use of 3D Laser Microscopy to Determine Plant Pollen. Preprints2021, 2021060699. https://doi.org/10.20944/preprints202106.0699.v1
Weber, A.; weber, K.; Pretot, S.; Sillipo, M.; Lombardi, L.; Warfving, N. Use of 3D Laser Microscopy to Determine Plant Pollen. Preprints 2021, 2021060699. https://doi.org/10.20944/preprints202106.0699.v1
Weber, A.; weber, K.; Pretot, S.; Sillipo, M.; Lombardi, L.; Warfving, N. Use of 3D Laser Microscopy to Determine Plant Pollen. Preprints2021, 2021060699. https://doi.org/10.20944/preprints202106.0699.v1
APA Style
Weber, A., weber, K., Pretot, S., Sillipo, M., Lombardi, L., & Warfving, N. (2021). Use of 3D Laser Microscopy to Determine Plant Pollen. Preprints. https://doi.org/10.20944/preprints202106.0699.v1
Chicago/Turabian Style
Weber, A., Leonardo Lombardi and Nils Warfving. 2021 "Use of 3D Laser Microscopy to Determine Plant Pollen" Preprints. https://doi.org/10.20944/preprints202106.0699.v1
Abstract
Pollen analysis as a part of palynology deals with the morphological determination of pollen and spores. Different technologies with different resolutions varying from simple light microscopy to highly elaborate electron microscopy are used for the examination, depending on the area of application (e.g. sedimentology, melissopalynology, forensic palynology, etc.). To answer the question of whether laser scanning microscopy (LSM) can replace scanning electron microscopy (SEM) for the determination of pollen species, 168 species were examined using LSM. It was concluded that LSM is both efficient and easy to handle. After preparing the fresh pollen, a 3D laser scan takes 5-10 minutes and unlike using SEM, the pollen does not have to be sputtered or processed. The 3D scans can be measured quickly and easily with the integrated software and there were no observable artifacts. At magnifications up to 8545x, the image quality is comparable to that of a sputtered SEM sample whereas at higher magnifications, the SEM method is superior. Overall, pollen display by LSM is much less time consuming and more cost effective than with the SEM method.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
