Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

mRNA inventory of extracellular vesicles from Ustilago maydis

Version 1 : Received: 8 June 2021 / Approved: 9 June 2021 / Online: 9 June 2021 (10:59:36 CEST)

A peer-reviewed article of this Preprint also exists.

Kwon, S.; Rupp, O.; Brachmann, A.; Blum, C.F.; Kraege, A.; Goesmann, A.; Feldbrügge, M. mRNA Inventory of Extracellular Vesicles from Ustilago maydis. J. Fungi 2021, 7, 562. Kwon, S.; Rupp, O.; Brachmann, A.; Blum, C.F.; Kraege, A.; Goesmann, A.; Feldbrügge, M. mRNA Inventory of Extracellular Vesicles from Ustilago maydis. J. Fungi 2021, 7, 562.

Journal reference: J. Fungi 2021, 7, 562
DOI: 10.3390/jof7070562

Abstract

Extracellular vesicles (EVs) can transfer diverse RNA cargo for intercellular signalling. EV-associated RNAs have been found in diverse fungi and were proposed to be relevant for pathogenesis in animal hosts. In plant-pathogen interactions, small RNAs are exchanged in a cross-kingdom RNAi warfare and EVs were considered to be a delivery mechanism. To extend the search for EV-associated molecules involved in plants-pathogen communication, we have characterised the repertoire of EV-associated mRNAs secreted by the maize smut pathogen, Ustilago maydis. For this initial survey, EVs were isolated from axenic filamentous cultures that mimic infectious hyphae. The EV-associated RNAs were resistant to degradation by RNases and the presence of intact mRNAs was evident. The set of mRNAs enriched inside EVs relative to the fungal cells are functionally distinct from those that are depleted from EVs, particularly overrepresented in metabolic enzyme activities. Intriguingly, mRNAs of some known effectors and other proteins linked to virulence were found in EVs. Furthermore, several mRNAs enriched in EVs are also upregulated during infection, suggesting that EV-associated mRNAs may participate in plant-pathogen interaction.

Subject Areas

Extracellular vesicles (EVs); mRNA; fungal pathogen; plant pathogen; Ustilago maydis

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