Version 1
: Received: 4 June 2021 / Approved: 7 June 2021 / Online: 7 June 2021 (08:01:21 CEST)
How to cite:
Haney, M.G.; Young, L.E.A.; Moore, L.H.; Chernyavskaya, Y.; Wei, M.; Markussen, K.H.; Ptacek, A.; Dockins, S.; Sanders, W.C.; Sun, R.C.; Blackburn, J.S. Optimization of Human Cancer Cell Xenograft Into Zebrafish Larvae for Anti-Cancer Drug Screening. Preprints2021, 2021060150
Haney, M.G.; Young, L.E.A.; Moore, L.H.; Chernyavskaya, Y.; Wei, M.; Markussen, K.H.; Ptacek, A.; Dockins, S.; Sanders, W.C.; Sun, R.C.; Blackburn, J.S. Optimization of Human Cancer Cell Xenograft Into Zebrafish Larvae for Anti-Cancer Drug Screening. Preprints 2021, 2021060150
Haney, M.G.; Young, L.E.A.; Moore, L.H.; Chernyavskaya, Y.; Wei, M.; Markussen, K.H.; Ptacek, A.; Dockins, S.; Sanders, W.C.; Sun, R.C.; Blackburn, J.S. Optimization of Human Cancer Cell Xenograft Into Zebrafish Larvae for Anti-Cancer Drug Screening. Preprints2021, 2021060150
APA Style
Haney, M.G., Young, L.E.A., Moore, L.H., Chernyavskaya, Y., Wei, M., Markussen, K.H., Ptacek, A., Dockins, S., Sanders, W.C., Sun, R.C., & Blackburn, J.S. (2021). Optimization of Human Cancer Cell Xenograft Into Zebrafish Larvae for Anti-Cancer Drug Screening. Preprints. https://doi.org/
Chicago/Turabian Style
Haney, M.G., Ramon C. Sun and Jessica S. Blackburn. 2021 "Optimization of Human Cancer Cell Xenograft Into Zebrafish Larvae for Anti-Cancer Drug Screening" Preprints. https://doi.org/
Abstract
Transplant of human cancer cells into zebrafish larvae has emerged as a useful methodology in cancer research. Zebrafish have very low husbandry costs, are amenable to large-scale drug screening, and are unmatched for optical clarity in live animal imaging. However, there is currently no consensus on the ideal methods for xenograft of human cancer cells into zebrafish. Here, we have examined the effects of transplant site and housing temperature on both zebrafish larvae and human cancer cells using survival analyses, metabolomic approaches, and in vivo imaging. Our data show that while zebrafish larvae can adapt to the ideal conditions for mammalian cells, human cancer cells are highly sensitive to both temperature change and transplant site. Human cells housed in slightly cooler than physiologic temperatures had a significantly altered metabolism that resulted in changes in growth, survival, and response to chemotherapy. Cancer cells xenografted into the yolk of the larvae also had reduced proliferation and drug response compared to those xenografted into the soma, in part due to the differences in metabolites available at these sites. In total, temperature and transplant site can have profound effects on xenografted cells. Standardization of zebrafish xenograft methods will enhance data reproducibility between individual laboratories.
Keywords
gas chromatography mass spectrometry; metabolomics; acute lymphoblastic leukemia; confocal microscopy; high-throughput drug screens; VAST
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
