Article
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Preserved in Portico This version is not peer-reviewed
Silica Nanoparticles Inhibit ATP Responses of Human Airway Epithelial 16HBE Cells
Version 1
: Received: 25 May 2021 / Approved: 26 May 2021 / Online: 26 May 2021 (11:45:50 CEST)
A peer-reviewed article of this Preprint also exists.
Milici, A.; Talavera, K.; Sanchez Linde, A. Silica Nanoparticles Inhibit Responses to ATP in Human Airway Epithelial 16HBE Cells. Biophysical Journal, 2023, 122, 392a. https://doi.org/10.1016/j.bpj.2022.11.2142.
Milici, A.; Talavera, K.; Sanchez Linde, A. Silica Nanoparticles Inhibit Responses to ATP in Human Airway Epithelial 16HBE Cells. Biophysical Journal, 2023, 122, 392a. https://doi.org/10.1016/j.bpj.2022.11.2142.
Abstract
Because of their low cost and easy production silica nanoparticles (NPs) are amply used in multiple manufactures as anti-caking, densifying and hydrophobic agents. However, this has increased the exposure levels of the general population and has raised concerns about possible toxicity of this nanomaterial. NPs are known to affect the function of the airway epithelium, but the biochemical pathways targeted by these particles remain largely unknown. Here we investigated the effects of NPs on the responses of cultured human bronchial epithelial (16HBE) cells to the damage-associated molecular pattern ATP, using fluorometric measurements of intracellular Ca2+ concentration. Upon stimulation with extracellular ATP these cells displayed a concentration-dependent increase in intracellular Ca2+, which was mediated by release from intracellular stores. Silica NPs inhibited the Ca2+ responses to ATP within minutes of application and at low micromolar concentrations, which are significantly faster and more potent than those previously reported for the induction of cellular toxicity and pro-inflammatory responses. NPs-induced inhibition appeared to be independent from the increase in intracellular Ca2+ they produce, and via a non-competitive mechanism. These findings suggest that NPs reduce the ability of airway epithelial cells to mount ATP-dependent protective responses such as the increase in mucociliary clearance and cough.
Keywords
silica; nanoparticles; ATP; purinergic receptor; airway; epithelial cell
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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