Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Microsatellites as Agents of Adaptive Change: An RNA-seq based Comparative Study of Transcriptomes from Five Helianthus Species

Version 1 : Received: 19 April 2021 / Approved: 20 April 2021 / Online: 20 April 2021 (08:21:06 CEST)

A peer-reviewed article of this Preprint also exists.

Ranathunge, C.; Pramod, S.; Renaut, S.; Wheeler, G.L.; Perkins, A.D.; Rieseberg, L.H.; Welch, M.E. Microsatellites as Agents of Adaptive Change: An RNA-Seq-Based Comparative Study of Transcriptomes from Five Helianthus Species. Symmetry 2021, 13, 933. Ranathunge, C.; Pramod, S.; Renaut, S.; Wheeler, G.L.; Perkins, A.D.; Rieseberg, L.H.; Welch, M.E. Microsatellites as Agents of Adaptive Change: An RNA-Seq-Based Comparative Study of Transcriptomes from Five Helianthus Species. Symmetry 2021, 13, 933.

Journal reference: Symmetry 2021, 13, 933
DOI: 10.3390/sym13060933

Abstract

Mutations that provide environment dependent selective advantages drive adaptive divergence among species. Many phenotypic differences among related species are more likely to result from gene expression divergence rather than from non-synonymous mutations. In this regard, cis-regulatory mutations play an important part in generating functionally significant variation. Some proposed mechanisms that explore the role of cis-regulatory mutations in gene expression divergence involve microsatellites. Microsatellites exhibit high mutation rates and are abundant in both coding and non-coding regions and could influence gene function and products. Here we tested the hypothesis that microsatellites contribute to gene expression divergence among species with 50 individuals from nine closely related Helianthus species using an RNA-seq approach. Differential expression analyses of the transcriptomes revealed that genes containing microsatellites in non-coding regions (UTRs and introns) are more likely to be differentially expressed among species when compared to genes with microsatellites in the coding regions and transcripts lacking microsatellites. We detected a greater proportion of shared microsatellites in 5’UTRs and coding regions compared to 3’UTRs and non-coding transcripts among Helianthus spp. Further, allele frequency differences measured by pairwise FST at single nucleotide polymorphisms (SNPs), indicate greater genetic divergence in transcripts containing microsatellites compared to those lacking microsatellites. A gene ontology (GO) analysis revealed that microsatellite-containing differentially expressed genes are significantly enriched for GO terms associated with regulation of transcription and transcription factor activity. Collectively, our study provides compelling evidence to support the role of microsatellites in gene expression divergence.

Subject Areas

Gene expression; Helianthus; microsatellites; transcriptomics

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