Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phase Correction for Absorption Mode Two-dimensional Mass Spectrometry

Version 1 : Received: 15 April 2021 / Approved: 16 April 2021 / Online: 16 April 2021 (12:41:15 CEST)

How to cite: Delsuc, M.; Breuker, K.; van Agthoven, M.A. Phase Correction for Absorption Mode Two-dimensional Mass Spectrometry. Preprints 2021, 2021040445 (doi: 10.20944/preprints202104.0445.v1). Delsuc, M.; Breuker, K.; van Agthoven, M.A. Phase Correction for Absorption Mode Two-dimensional Mass Spectrometry. Preprints 2021, 2021040445 (doi: 10.20944/preprints202104.0445.v1).

Abstract

Two-dimensional mass spectrometry (2D MS) is a method for tandem mass spectrometry that relies on manipulating ion motions to correlate precursor and fragment ion signals. 2D mass spectra are obtained by performing a Fourier transform in both the precursor ion mass-to-charge ratio (m/z) dimension and the fragment ion m/z dimension. The phase of the ion signals evolves linearly in the precursor m/z dimension and quadratically in the fragment m/z dimension. This study demonstrates that phase-corrected absorption mode 2D mass spectrometry improves signal-to-noise ratios by a factor of 2 and resolving power by a factor of 2 in each dimension compared to magnitude mode. Furthermore, phase correction leads to an easier differentiation between ion signals and artefacts, and therefore easier data interpretation.

Subject Areas

mass spectrometry; two-dimensional; tandem mass spectrometry; phase correction; absorption mode; data processing

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