Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Exosomes Derived from SIRT6 Transfected Urine-Derived Stem Cells Improves Renal Fibrosis

Version 1 : Received: 17 March 2021 / Approved: 18 March 2021 / Online: 18 March 2021 (10:57:54 CET)

How to cite: Wang, R. Exosomes Derived from SIRT6 Transfected Urine-Derived Stem Cells Improves Renal Fibrosis. Preprints 2021, 2021030472 (doi: 10.20944/preprints202103.0472.v1). Wang, R. Exosomes Derived from SIRT6 Transfected Urine-Derived Stem Cells Improves Renal Fibrosis. Preprints 2021, 2021030472 (doi: 10.20944/preprints202103.0472.v1).

Abstract

Purpose: The treatment of renal fibrosis caused by long-term obstructive nephropathy is limited. The purpose of this study was to establish a mouse model of renal fibrosis with unilateral ureteral obstruction (UUO) and to treat it with exosomes derived from SIRT6 transfected urine-derived stem cells (USCs), which will determine whether exosomes have the effect of anti-fibrosis Methods: The renal fibrosis model of UUO mice was established by ligating unilateral ureter. USCs were extracted from human urine and transfected with SIRT6 lentivirus plasmid. The SIRT6-USCs-exosomes(SIRT6-USCs-exos) were collected and identified by transmission electron microscope, nanoparticle tracking analysis and western blot. SIRT6-USCs-exos were injected into the tail vein of mice and the renal tissue of mice was stained with HE. The relative gene expressions of α-SMA, E-cadherin and TGF-β1 were analyzed by RT-qPCR. Results: In this study, we successfully constructed the renal fibrosis model of UUO mice and hUSCs with overexpression of SIRT6. The SIRT6-USCs-exos significantly improved the renal fibrosis in UUO mice. The relative mRNA expressions of fibrosis-related genes α-SMA, E-cadherin and TGF-β1 in renal tissue were significantly down-regulated after SIRT6-USCs-exos treatment. Conclusion: Our results indicate that SIRT6-USCs-exos can effectively alleviate renal fibrosis caused by long-term obstructive nephropathy. The findings may be promising for dealing with renal fibrosis.

Subject Areas

renal fibrosis; USCs; exosome; SIRT6

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