Version 1
: Received: 16 March 2021 / Approved: 17 March 2021 / Online: 17 March 2021 (16:55:21 CET)
How to cite:
Rashid, M.; Shamshad, A.; Jankuloski, L. Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice. Preprints2021, 2021030453. https://doi.org/10.20944/preprints202103.0453.v1
Rashid, M.; Shamshad, A.; Jankuloski, L. Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice. Preprints 2021, 2021030453. https://doi.org/10.20944/preprints202103.0453.v1
Rashid, M.; Shamshad, A.; Jankuloski, L. Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice. Preprints2021, 2021030453. https://doi.org/10.20944/preprints202103.0453.v1
APA Style
Rashid, M., Shamshad, A., & Jankuloski, L. (2021). Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice. Preprints. https://doi.org/10.20944/preprints202103.0453.v1
Chicago/Turabian Style
Rashid, M., Areeqa Shamshad and Ljupcho Jankuloski. 2021 "Effects of Ethyl methanesulfonate (EMS) on Seedling and Yield Contributing Traits in Basmati Rice" Preprints. https://doi.org/10.20944/preprints202103.0453.v1
Abstract
Increasing genetic diversity in crop plants has been used for chemical mutagenesis. Through the application of various mutagenic agents, over 430 new varieties have been derived as rice mutants (Oryza sativa L.) Chemical mutagens such as ethyl methane sulphonate (EMS), diepoxybutane derivative (DEB), sodium azide, and gamma ray, x-ray, and quick neutron irradiation have been commonly used to induce a large number of functional variations in rice and others crops. Among chemical mutagens, ethyl methane sulfonate (EMS) is the alkylating agent most widely used in plants because it induces nucleotide substitutions to be extremely frequent, as detected in various genomes. In this study, seeds of potential genotype of the popular variety, (Oryza sativa L. Super Basmati variety) were treated with EMS at concentrations of 0.25%, 0.50%, 0.75%, 1% and 1.5%. Various measurements on the M1 generation determined EMS sensitivity. As concentration of applied EMS increased, will decrease in germination, shoot length, root length, plant height, productive tillers, Panicle Length, Total Spikelet, sterile spikelet and fertility under field conditions were observed in M1 generation as compared to the non-treatment control. Emergence, shoot length, root length, plant height, productive tillers, Panicle Length, Total Spikelet, sterile spikelet and fertility also decreased with increases in EMS mutagenesis in an approximately linear fashion. The LD50 values were observed based on growth reduction of seedlings after EMS treatment with 0.25% and 0.50% on the rice variety (Oryza sativa L. spp.).
Keywords
Lethal Dose; Chemical Mutagenesis; Ethyl methanesulfonate; Oryza Sativa L
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
