Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Determining the Cytotoxic Properties and Mechanisms Against Drug Resistance of an Aqueous Extract of Fagonia indica on Colon Cancer Cells

Version 1 : Received: 26 February 2021 / Approved: 4 March 2021 / Online: 4 March 2021 (14:48:30 CET)

How to cite: Lewis, A.; Shelton, C.; Wang, W.; Kannappan, V.; Carmichael, A.R.; Rothnie, A.; Brown, J.E. Determining the Cytotoxic Properties and Mechanisms Against Drug Resistance of an Aqueous Extract of Fagonia indica on Colon Cancer Cells. Preprints 2021, 2021030168 (doi: 10.20944/preprints202103.0168.v1). Lewis, A.; Shelton, C.; Wang, W.; Kannappan, V.; Carmichael, A.R.; Rothnie, A.; Brown, J.E. Determining the Cytotoxic Properties and Mechanisms Against Drug Resistance of an Aqueous Extract of Fagonia indica on Colon Cancer Cells. Preprints 2021, 2021030168 (doi: 10.20944/preprints202103.0168.v1).

Abstract

Fagonia indica is a perennial plant grown in arid climates and utilised in traditional medicine as a treatment for cancer. Previous research has highlighted potential antineoplastic effects of Fagonia indica against breast cancer cell lines. Despite this, there has been little research demonstrating the potential of an aqueous extract of Fagonia indica against colon cancer. Colon cancer is a leading cause of cancer-related mortality, with drug resistance remaining a barrier to treatment. The aim of the present study was to investigate the cytotoxic mechanisms of Fagonia indica in colon cancer cells, including pathways related to proliferation, angiogenesis and inflammation, and ABC membrane transporters. Treatment with Fagonia indica caused a reduction in cell viability in wild-type and chemotherapy resistant colon cancer. Results indicated a role for Akt/ MAPK signalling as a mechanism of Fagonia indica induced cell death, alongside a reduction in the expression of VEG-F, NK-κB and ICAM-1. RT-PCR demonstrated a reduction in expression of ABCG2/ABCC4 in resistant colon cancers, after treatment with Fagonia indica extract. Further research is required to isolate bioactive compounds of Fagonia indica for use against colon cancer.

Subject Areas

Fagonia indica, colon cancer, proliferation, angiogenesis, ABCC4, ABCG2

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