Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genome Mining and Heterologous Expression of Lipoamides and Amicoumacins From Compost-Associated Bacillus subtilis fmb60

jie yang
ORCID logo ,
Qingzheng Zhu
,
Feng Xu
,
Ming Yang
,
Hechao Du
,
Xiaoying Bian
ORCID logo ,
Zhaoxin Lu
ORCID logo ,
Yingjian Lu
* ,
Fengxia Lu
Version 1 : Received: 2 March 2021 / Approved: 3 March 2021 / Online: 3 March 2021 (14:08:28 CET)

A peer-reviewed article of this Preprint also exists.

Yang, J.; Zhu, Q.; Xu, F.; Yang, M.; Du, H.; Bian, X.; Lu, Z.; Lu, Y.; Lu, F. Genome Mining, Heterologous Expression, Antibacterial and Antioxidant Activities of Lipoamides and Amicoumacins from Compost-Associated Bacillus subtilis fmb60. Molecules 2021, 26, 1892. Yang, J.; Zhu, Q.; Xu, F.; Yang, M.; Du, H.; Bian, X.; Lu, Z.; Lu, Y.; Lu, F. Genome Mining, Heterologous Expression, Antibacterial and Antioxidant Activities of Lipoamides and Amicoumacins from Compost-Associated Bacillus subtilis fmb60. Molecules 2021, 26, 1892.

Abstract

Abstract: Bacillus subtilis fmb60, which has broad-spectrum antimicrobial activities was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Three lipoamides D–F (1-3) and two amicoumacin derivatives, amicoumacins D, E (4, 5), were identified, and are reported here for the first time. Lipoamides D–F exhibited strong antibacterial activities against harmful foodborne bacteria, with the MIC ranging from 6.25 to 25 µg/mL. Amicoumacin E scavenged 38.8% of ABTS+ radicals at 1 mg/mL. Direct cloning and heterologous expression of the NRPS/PKS and ace gene cluster identified its importance for the biosynthesis of amicoumacins. This study demonstrated that there is a high potential for biocontrol utilization of B. subtilis fmb60, and genome mining for clusters of secondary metabolites of B. subtilis fmb60 has revealed a greater biosynthetic potential for the production of novel natural products than previously anticipated.

Keywords

Bacillus subtilis; NRPS/PKS; amicoumacins; heterologous expression; bioactivities

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.