Version 1
: Received: 9 February 2021 / Approved: 12 February 2021 / Online: 12 February 2021 (15:12:52 CET)
How to cite:
Costas-Malvido, M.; Astray, G.; Mejuto, J.C.; Bendaña-Jácome, R.J.; Pérez-Guerra, N. Nisin Synthesis, Relationship with the Growth of the Producing Strain, Culture pH and Nitrogen Consumption. Preprints2021, 2021020311. https://doi.org/10.20944/preprints202102.0311.v1
Costas-Malvido, M.; Astray, G.; Mejuto, J.C.; Bendaña-Jácome, R.J.; Pérez-Guerra, N. Nisin Synthesis, Relationship with the Growth of the Producing Strain, Culture pH and Nitrogen Consumption. Preprints 2021, 2021020311. https://doi.org/10.20944/preprints202102.0311.v1
Costas-Malvido, M.; Astray, G.; Mejuto, J.C.; Bendaña-Jácome, R.J.; Pérez-Guerra, N. Nisin Synthesis, Relationship with the Growth of the Producing Strain, Culture pH and Nitrogen Consumption. Preprints2021, 2021020311. https://doi.org/10.20944/preprints202102.0311.v1
APA Style
Costas-Malvido, M., Astray, G., Mejuto, J.C., Bendaña-Jácome, R.J., & Pérez-Guerra, N. (2021). Nisin Synthesis, Relationship with the Growth of the Producing Strain, Culture pH and Nitrogen Consumption. Preprints. https://doi.org/10.20944/preprints202102.0311.v1
Chicago/Turabian Style
Costas-Malvido, M., Ricardo J. Bendaña-Jácome and Nelson Pérez-Guerra. 2021 "Nisin Synthesis, Relationship with the Growth of the Producing Strain, Culture pH and Nitrogen Consumption" Preprints. https://doi.org/10.20944/preprints202102.0311.v1
Abstract
Nisin, an antibacterial compound produced by Lactococcus lactis strains, has been approved by the US Food and Drug Administration to be used as a safe food additive to control the growth of undesirable pathogenic bacteria. Nisin is commonly described as a pH-dependent primary metabolite, since its production depends on growth and culture pH evolution. However, the relationship between bacteriocin synthesis and the consumption of the limiting nutrient has not been described until now. Therefore, this study aimed to develop a competitive four-dimensional Lotka Volterra-like equation to describe the relationships between culture pH, limiting nutrient (total nitrogen: TN) consumption and production of biomass (X) and nisin (BT) in four series of batch fermentation with L. lactis CECT 539 in diluted whey (DW)-based media. The developed four-dimensional LV-like equation (with a unique set of parameters) could not be used to describe all cultures belonging to each fermentation series. However, the four-dimensional LV-like equation described accurately each individual culture, providing a good description of the relationships between pH, TN, X and BT, higher values for R2 and F-ratios, lower values (< 10%) for the mean relative percentage deviation modulus, with bias and accuracy factor values approximately equal to one.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
*
