Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Growth Promotion Function of Bacillus sp. Strains Isolated From Salt-Pan Rhizosphere and Their Biocontrol Potential Against Macrophomina Phaseolina

Stefany Castaldi
,
Claudia Petrillo
ORCID logo ,
Giuliana Donadio
,
Fabrizio del Piaz
,
Alessio Cimmino
ORCID logo ,
Marco Masi
ORCID logo ,
Antonio Evidente
ORCID logo ,
Rachele Isticato
*
Version 1 : Received: 8 February 2021 / Approved: 9 February 2021 / Online: 9 February 2021 (15:09:32 CET)

A peer-reviewed article of this Preprint also exists.

Castaldi, S.; Petrillo, C.; Donadio, G.; Piaz, F.D.; Cimmino, A.; Masi, M.; Evidente, A.; Isticato, R. Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina. Int. J. Mol. Sci. 2021, 22, 3324. Castaldi, S.; Petrillo, C.; Donadio, G.; Piaz, F.D.; Cimmino, A.; Masi, M.; Evidente, A.; Isticato, R. Plant Growth Promotion Function of Bacillus sp. Strains Isolated from Salt-Pan Rhizosphere and Their Biocontrol Potential against Macrophomina phaseolina. Int. J. Mol. Sci. 2021, 22, 3324.

Abstract

In the last decades, intensive crop management has involved excessive use of pesticides or fertilizers, compromising environmental integrity and public health. Accordingly, there has been worldwide pressure to find an eco-friendly and safe strategy to ensure agricultural productivity. Recently, Plant Growth-Promoting (PGP) rhizobacteria are receiving increasing attention as suitable biocontrol agents against agricultural pests. In the present study, 22 spore-forming bacteria were selected among a salt-pan rhizobacteria collection for their PGP traits and their antagonistic activity against the plant pathogen fungus Macrophomina phaseolina. Based on the higher antifungal activity, strain RHFS10, identified as Bacillus vallismortis, was furtherly examined and cell-free supernatants assays, column purification, and tandem mass spectrometry employed to purify and preliminarily identify the antifungal metabolites. Interestingly, the minimum inhibitory concentration assessed for the fractions active against M. phaseolina, resulted 10 times lower and more stable than the one estimated for the commercial fungicide pentachloronitrobenzene. These results suggest the use of B. vallismortis strain RHFS10 as a potential Plant Growth Promoting Rhizobacteria to efficiently control phytopathogenic fungus M. phaseolina, in alternative to chemical pesticides.

Keywords

plant-growth-promoting bacteria; spore-forming bacteria; Bacillus vallismortis; Macrophomina phaseolina; phenotypic and genotypic characterization; Biocontrol agents

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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