Article
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At the molecular resolution with MINFLUX?
Version 1
: Received: 5 February 2021 / Approved: 8 February 2021 / Online: 8 February 2021 (07:50:16 CET)
How to cite: Prakash, K. At the molecular resolution with MINFLUX?. Preprints 2021, 2021020173. https://doi.org/10.20944/preprints202102.0173.v1 Prakash, K. At the molecular resolution with MINFLUX?. Preprints 2021, 2021020173. https://doi.org/10.20944/preprints202102.0173.v1
Abstract
Gwosch et al. (2020) and Balzarotti et al. (2017) purport MINFLUX as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1-3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether true 1 nm resolution has been attained. Here, I compare the performance with other super-resolution methods focussing particularly on spatial resolution claims, atypical image rendering, visualisation enhancement, subjective filtering of localizations, detection vs labelling efficiency and the possible limitations when imaging biological samples containing densely labelled structures. I hope the analysis and evaluation parameters presented here are not only useful for future research directions but also microscope users, developers and core facility managers when deciding on an investment for the next 'state-of-the-art' instrument.
Keywords
MINFLUX, STED, SMLM, localisation precision, image resolution, super-resolution imaging
Subject
Biology and Life Sciences, Biophysics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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