Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Could Protein Content of Urinary Extracellular Vesicles be Useful to Detect Alcoholic Liver Disease and Assess Hepatocarcinoma Risk?

Version 1 : Received: 31 January 2021 / Approved: 1 February 2021 / Online: 1 February 2021 (18:15:22 CET)

How to cite: E., G.; M., A.; C., G.; J., P.; F., E.; S., B.; JM, F. Could Protein Content of Urinary Extracellular Vesicles be Useful to Detect Alcoholic Liver Disease and Assess Hepatocarcinoma Risk?. Preprints 2021, 2021020069 (doi: 10.20944/preprints202102.0069.v1). E., G.; M., A.; C., G.; J., P.; F., E.; S., B.; JM, F. Could Protein Content of Urinary Extracellular Vesicles be Useful to Detect Alcoholic Liver Disease and Assess Hepatocarcinoma Risk?. Preprints 2021, 2021020069 (doi: 10.20944/preprints202102.0069.v1).

Abstract

(1) Background: Alcohol abuse has a high impact on the mortality and morbidity related to a great number of diseases and is responsible for the development of alcoholic liver disease (ALD). It remains challenging to detect and evaluate its severity, which is crucial for prognosis. In this work, we studied if urinary EVs (uEVs) could serve in diagnose and evaluate cirrhosis in ALD. (2) Methods: uEVs characterization by cryo-electron microscopy (Cryo-EM), Nanoparticle Tracking Analysis (NTA) and Western blotting (WB) was performed in a cohort of 21 controls and 21 cirrhotic patients. Then, proteomics of urinary EVs (uEVs) was carried out in a second cohort of 6 controls and 8 patients in order to identify new putative biomarkers for cirrhosis in ALD. (3) Results: uEVs concentration, size and composition were altered in cirrhotic patients. A total of 1304 proteins were identified in uEVs, and 90 of them were found to be altered in cirrhotic patients. (4) Conclusions: uEVs could be considered as a tool and a supplier of new biomarkers for ALD, whose application would be especially relevant in chronic patients. Yet, further research is necessary to obtain more relevant result in clinical terms.

Subject Areas

extracellular vesicles (EVs); urinary extracellular vesicles (uEVs); exosomes; biomarkers; liquid biopsy; cirrhosis; fibrosis; hepatocarcinoma (HCC); alcoholic liver disease (ALD).

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