Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Intensification of Biokinetics of Enzymes using Ultrasound Assisted Methods: A Critical Review

Version 1 : Received: 9 January 2021 / Approved: 11 January 2021 / Online: 11 January 2021 (12:49:39 CET)

How to cite: Khan, A.; Beg, M.R.; Waghmare, P. Intensification of Biokinetics of Enzymes using Ultrasound Assisted Methods: A Critical Review. Preprints 2021, 2021010194 (doi: 10.20944/preprints202101.0194.v1). Khan, A.; Beg, M.R.; Waghmare, P. Intensification of Biokinetics of Enzymes using Ultrasound Assisted Methods: A Critical Review. Preprints 2021, 2021010194 (doi: 10.20944/preprints202101.0194.v1).

Abstract

The use of low-intensity ultrasound has gotten surprising consideration over the last decade as a method for enhancing the catalytic activity of the enzyme. Ultrasounds have the potential to significantly influence the activity of the enzymatic processes, provided that the energy input is not too high to inactivate the enzyme. By providing the variation in parameters, various physical and chemical effects can be attained that can enhance the enzymatic reaction. Ultrasonic reactors are known for their application in bioprocesses. However, the potential of their applications is still limited broadly due to the lack of proper information about their operational and performance parameters. In this review, the detailed information about ultrasonic reactors is provided by defining the different types of reactors, number and position of ultrasonic transducers. Also, it includes the mechanism of intensification and influence of ultrasonic parameters (intensity, duty cycle and frequency) and enzymatic factors (enzyme concentration, temperature and pH) on the catalytic activity of enzyme during ultrasound treatment.

Keywords

ultrasound; ultrasonic reactors; enzyme kinetic; enzymatic reaction; sonochemical reaction

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