Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Establishment of a Sheep Model for Hind limb Peripheral Nerve Injury: Common Peroneal Nerve

Rui D. Alvites
ORCID logo ,
Mariana V. Branquinho
ORCID logo ,
Ana Catarina Sousa
,
Federica Zen
,
Monica Maurina
,
Stefania Raimondo
ORCID logo ,
Carla Mendonça
,
Luís Miguel Atayde
,
Stefano Geuna
ORCID logo ,
Artur S. P. Varejão
ORCID logo ,
Ana Colette Maurício
* ORCID logo
Version 1 : Received: 21 December 2020 / Approved: 22 December 2020 / Online: 22 December 2020 (16:07:28 CET)

A peer-reviewed article of this Preprint also exists.

Alvites, R.D.; Branquinho, M.V.; Sousa, A.C.; Zen, F.; Maurina, M.; Raimondo, S.; Mendonça, C.; Atayde, L.; Geuna, S.; Varejão, A.S.P.; Maurício, A.C. Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve. Int. J. Mol. Sci. 2021, 22, 1401. Alvites, R.D.; Branquinho, M.V.; Sousa, A.C.; Zen, F.; Maurina, M.; Raimondo, S.; Mendonça, C.; Atayde, L.; Geuna, S.; Varejão, A.S.P.; Maurício, A.C. Establishment of a Sheep Model for Hind Limb Peripheral Nerve Injury: Common Peroneal Nerve. Int. J. Mol. Sci. 2021, 22, 1401.

Abstract

Thousands of people worldwide suffer from injuries in the peripheral nerve and deal daily with the resulting physiological and functional deficits. Recent advances in this field are still insufficient to guarantee effective outcomes, and the development of new and effective therapeutic options requires the use of valid preclinical models that effectively replicate the characteristics and challenges associated with these injuries in humans. In this study, we established a sheep model for common peroneal nerve injuries that can be applied in preclinical research with the advantages associated with the use of large animal models. In an integrative way, this article includes a detailed description of the anatomy and functionality of the peripheral nerves of sheep’s hind limb, the surgical protocol for accessing the common peroneal nerve, the induction of different types of nerve damage and the application of possible therapeutic options. A neurological exam protocol directed to the common peroneal nerve was also established, allowing to identify the changes and deficits related with the nerve injury and to evaluate the functional progression over time. Finally, a preliminary stereological study was carried out to establish control values for the healthy peroneal common nerves of this model and to identify preliminary differences between therapeutic methods. The ultimate goal is to demonstrate that sheep is a valid model of peripheral nerve injury to be used in pre-clinical and translational works and to evaluate the efficacy and safety of nerve injury therapeutic options before its clinical application in human and veterinary patients.

Keywords

Peripheral Nerve Injury; Peripheral Nerve Regeneration; Peroneal Common Nerve; Animal Model; Sheep Model; Nerve Anatomy; Neurological Exam; Nerve Stereology

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.