PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Identification of a Novel Interaction Between SMC1 DNA Damage Repair Protein and Escherichia Coli O157: H7 EspF Using Co-Immunoprecipitation Combined With Mass Spectrometry
Version 1
: Received: 20 December 2020 / Approved: 22 December 2020 / Online: 22 December 2020 (10:33:53 CET)
How to cite:
Fu, M.; Hua, Y.; Wu, J.; Zhang, Z.; Liu, J.; Li, X.; Fang, Y.; Zhang, B.; Zhao, W.; Wan, C. Identification of a Novel Interaction Between SMC1 DNA Damage Repair Protein and Escherichia Coli O157: H7 EspF Using Co-Immunoprecipitation Combined With Mass Spectrometry. Preprints2020, 2020120551. https://doi.org/10.20944/preprints202012.0551.v1
Fu, M.; Hua, Y.; Wu, J.; Zhang, Z.; Liu, J.; Li, X.; Fang, Y.; Zhang, B.; Zhao, W.; Wan, C. Identification of a Novel Interaction Between SMC1 DNA Damage Repair Protein and Escherichia Coli O157: H7 EspF Using Co-Immunoprecipitation Combined With Mass Spectrometry. Preprints 2020, 2020120551. https://doi.org/10.20944/preprints202012.0551.v1
Fu, M.; Hua, Y.; Wu, J.; Zhang, Z.; Liu, J.; Li, X.; Fang, Y.; Zhang, B.; Zhao, W.; Wan, C. Identification of a Novel Interaction Between SMC1 DNA Damage Repair Protein and Escherichia Coli O157: H7 EspF Using Co-Immunoprecipitation Combined With Mass Spectrometry. Preprints2020, 2020120551. https://doi.org/10.20944/preprints202012.0551.v1
APA Style
Fu, M., Hua, Y., Wu, J., Zhang, Z., Liu, J., Li, X., Fang, Y., Zhang, B., Zhao, W., & Wan, C. (2020). Identification of a Novel Interaction Between SMC1 DNA Damage Repair Protein and <em>Escherichia Coli</em> O157: H7 EspF Using Co-Immunoprecipitation Combined With Mass Spectrometry. Preprints. https://doi.org/10.20944/preprints202012.0551.v1
Chicago/Turabian Style
Fu, M., Wei Zhao and Chengsong Wan. 2020 "Identification of a Novel Interaction Between SMC1 DNA Damage Repair Protein and <em>Escherichia Coli</em> O157: H7 EspF Using Co-Immunoprecipitation Combined With Mass Spectrometry" Preprints. https://doi.org/10.20944/preprints202012.0551.v1
Abstract
The enterohemorrhagic Escherichia coli (EHEC) O157: H7 EspF is known to be a multifunctional effector that triggers several damage processes in the host cells. However, in the process of EHEC O157: H7 infection, the interactions between EspF, its N- or C-terminus, and host proteins are still unclear. In this work, we use co-immunoprecipitation combined with mass spectrometry (CoIP-MS) to screen the interactions between EspF/EspF-N/EspF-C terminus and host proteins. A total of 311 host proteins are analyzed. The N-terminus of EspF is found to interact with 192 proteins, whereas 205 proteins interact with the C-terminus. These proteins are mainly involved in RNA splicing, endoplasmic reticulum stress, and a variety of metabolic signaling pathways. Surprisingly, MS results reveal that EspF can also phosphorylate H2AX, suggesting that EspF may directly mediate DNA damage. Here, by western blot and immunofluorescence (IF), we verified that EspF can cause phosphorylation of H2AX and mediates cell multi-nuclearation and cell hypertrophy. Furthermore, we verify here for the first time that SMC1 interacts with EspF -C-terminus, and provide evidence that EspF increases p-SMC1 levels. p-SMC1 is known to influence S-phase cell cycle arrest and usually increases during periods of DNA damage. Our work revealed a novel interaction between EspF and the host protein SMC1 and lays a foundation for further research on EspF-mediated host DNA damage, apoptosis, and even colorectal carcinogenesis.
Keywords
Enterohemorrhagic Escherichia coli O157: H7; EspF; SMC1; CoIP-MS; DNA damage
Subject
Biology and Life Sciences, Anatomy and Physiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
