Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing

Søren Iversen
* ORCID logo ,
Thor Bech Johannesen
,
Anna Cäcilia Ingham
,
Sofie Marie Edslev
,
Staffan Tevell
ORCID logo ,
Emeli Månsson
,
Åsa Nilsdotter-Augustinsson
,
Bo Söderquist
ORCID logo ,
Marc Stegger
,
Paal Skytt Andersen
Version 1 : Received: 24 November 2020 / Approved: 25 November 2020 / Online: 25 November 2020 (12:56:25 CET)

A peer-reviewed article of this Preprint also exists.

Iversen, S.; Johannesen, T.B.; Ingham, A.C.; Edslev, S.M.; Tevell, S.; Månsson, E.; Nilsdotter-Augustinsson, Å.; Söderquist, B.; Stegger, M.; Andersen, P.S. Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing. Microorganisms 2020, 8, 1977. Iversen, S.; Johannesen, T.B.; Ingham, A.C.; Edslev, S.M.; Tevell, S.; Månsson, E.; Nilsdotter-Augustinsson, Å.; Söderquist, B.; Stegger, M.; Andersen, P.S. Alteration of Bacterial Communities in Anterior Nares and Skin Sites of Patients Undergoing Arthroplasty Surgery: Analysis by 16S rRNA and Staphylococcal-Specific tuf Gene Sequencing. Microorganisms 2020, 8, 1977.

Abstract

The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty to assess the impact of chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection of samples obtained from elective arthroplasty patients (n=83) by swabbing anterior nares, skin sites in the groin and the site of planned surgery, before and after arthroplasty surgery, was analysed by 16S rRNA (V3-V4) gene sequencing and a complementary targeted tuf gene sequencing approach to comprehensively characterise alterations in staphylococcal communities. Significant reductions in alpha diversity was detected for both bacterial (p=0.04) and staphylococcal (p=0.03) groin communities after arthroplasty surgery with significant reductions in relative Corynebacterium (p=0.001) abundance and S. hominis (p=0.01) relative staphylococcal abundance. In nares, significant reductions occurred for S. hominis (p=0.02), S. haemolyticus (p=0.02), and S. pasteuri (p=0.003) relative to other staphylococci. S. aureus colonised 35% of anterior nares before and 26% after arthroplasty surgery. S. epidermidis was the most abundant staphylococcal species at all sampling sites. No bacterial genus or staphylococcal species increased significantly after arthroplasty surgery. Application of a targeted tuf gene sequencing approach provided auxiliary staphylococcal community profiles and allowed species-level characterisation directly from low biomass clinical samples.

Keywords

Arthroplasty; prosthetic joint replacement; prosthetic joint infection; systemic antibiotic prophylaxis; Antibiotics; chlorhexidine gluconate; coagulase-negative staphylococci; tuf gene sequencing; staphylome; microbiome

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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